2. Metabolic Enzyme/Protease NF-κB Autophagy
  3. Phosphodiesterase (PDE) Endogenous Metabolite NF-κB Autophagy
  4. Moracin M

Moracin M is a phenolic component that can be isolated from Mori Cortex, is a potent phosphodiesterase-4 (PDE4) inhibitor with IC50 values of 2.9, 4.5, >40, and >100 μM for PDE4D2, PDE4B2, PDE5A1, and PDE9A2, respectively. Moracin M has anti-inflammatory activity.

For research use only. We do not sell to patients.

Moracin M Chemical Structure

Moracin M Chemical Structure

CAS No. : 56317-21-6

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10 mM * 1 mL in DMSO
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Customer Review

Based on 1 publication(s) in Google Scholar

Moracin M Related Antibodies

Top Publications Citing Use of Products

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Moracin M is a phenolic component that can be isolated from Mori Cortex, is a potent phosphodiesterase-4 (PDE4) inhibitor with IC50 values of 2.9, 4.5, >40, and >100 μM for PDE4D2, PDE4B2, PDE5A1, and PDE9A2, respectively. Moracin M has anti-inflammatory activity[1][2].

IC50 & Target[1]

PDE4D2

2.9 μM (IC50)

PDE4B2

4.5 μM (IC50)

PDE5A1

>40 μM (IC50)

PDE9A2

>100 μM (IC50)

In Vitro

Moracin M (30, 60 μM, 2h) inhibits the expression of IL-6 in A549 cells by inhibiting the activation of JNK/c-Jun. The IC50 value of Moracin M for IL-6 is 8.1 μM[1].
Moracin M inhibits the production of NO by inhibiting the expression of iNOS in alveolar macrophages (MH-S). The IC50 value of Moracin M for NO is 65.7 μM[2].
Moracin M (LPS 0.5 μg/mL, Moracin M 5-20 μM; 24h) can effectively inhibits the inflammatory response induced by lipopolysaccharide (LPS (HY-D1056)) in the intervertebral disc nucleus pulposus cell (NPCs) model, specifically by reducing the increase of inflammatory factors IL-1β, TNF-α and IL-6 [3].
Moracin M (LPS 0.5 μg/mL, Moracin M 5-20 μM; 24h) promots the autophagy and inhibits the production of inflammatory mediators in NPCs model by inhibiting the phosphorylation of PI3K and Akt [3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Moracin M Related Antibodies

RT-PCR[2]

Cell Line: A549 human lung epithelial cell line
Concentration: 30, 60 μM
Incubation Time: 2h
Result: Inhibited the activation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK)

Western Blot Analysis[2]

Cell Line: A549 human lung epithelial cell line
Concentration: 30, 60 μM
Incubation Time:
Result: Clearly inhibited IL-6 expression in IL-1β-treated lung epithelial cells.
The expression of iNOS was significantly inhibited.

Western Blot Analysis[2]

Cell Line: A549 human lung epithelial cell line
Concentration: 30, 60 μM
Incubation Time:
Result: Clearly inhibited IL-6 expression in IL-1β-treated lung epithelial cells.
The expression of iNOS was significantly inhibited.

Western Blot Analysis[2]

Cell Line: lipopolysaccharide (LPS)-treated nucleus pulposus cells (NPCs)
Concentration: LPS (HY-D1056) 0.5 μg/mL , Moracin M 5-20 μM
Incubation Time: 24h
Result: Effectively inhibited lipopolysaccharide (LPS) -induced inflammatory response, specifically by reducing the increase of inflammatory cytokines IL-1β, TNF-α and IL-6
Inhibited the phosphorylation of PI3K and Akt, promoted the autophagy of NPCs (autophagy) and inhibited the production of inflammatory mediators
In Vivo

Moracin M (20, 60 mg/kg, Oral gavage) reduces inflammatory response in LPS (HY-D1056) -induced acute lung injury (ALI) mouse models, decreases the total number of infiltrated cells in bronchoalveolar lavage (BAL) fluid, and significantly reduces the number of neutrophils, macrophages and dendritic cells[2].
Moracin M (20, 60 mg/kg, Oral gavage) alleviates pathological changes in lung tissue in ALI mouse models, including reduces alveolar wall thickness, increases alveolar space, and reduces inflammatory cell infiltration in lung tissue[2].
Moracin M (20, 60 mg/kg, Oral gavage) significantly inhibits NF-κB activation in the lungs in a mouse model of ALI[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: LPS-induced acute lung injury (ALI) mouse models[2]
Dosage: LPS (HY-D1056) 2 mg/kg; LPS 2 mg/kg+ Moracin M 20 mg/kg, 60 mg/kg; LPS 2 mg/kg+ Dexamethasone (HY-14648) 30 mg/kg. (n=13)
Administration: Oral gavage
Result: Reduced the inflammatory response in ALI mouse models and reduced the total number of cells infiltrated in bronchoalveolar lavage (BAL) fluid. The number of neutrophils, macrophages and dendritic cells was significantly decreased.
Reduced the thickness of alveolar wall, increasing alveolar space, and reduced the infiltration of inflammatory cells in lung tissue.
Molecular Weight

242.23

Formula

C14H10O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

OC1=CC(C2=CC3=CC=C(O)C=C3O2)=CC(O)=C1
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (68.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1283 mL 20.6415 mL 41.2831 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

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Concentration (final)

C2

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Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.67 mg/mL (6.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.67 mg/mL (6.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.67 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.90%

COA (248 KB) LCMS (265 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1283 mL 20.6415 mL 41.2831 mL 103.2077 mL
5 mM 0.8257 mL 4.1283 mL 8.2566 mL 20.6415 mL
10 mM 0.4128 mL 2.0642 mL 4.1283 mL 10.3208 mL
15 mM 0.2752 mL 1.3761 mL 2.7522 mL 6.8805 mL
20 mM 0.2064 mL 1.0321 mL 2.0642 mL 5.1604 mL
25 mM 0.1651 mL 0.8257 mL 1.6513 mL 4.1283 mL
30 mM 0.1376 mL 0.6881 mL 1.3761 mL 3.4403 mL
40 mM 0.1032 mL 0.5160 mL 1.0321 mL 2.5802 mL
50 mM 0.0826 mL 0.4128 mL 0.8257 mL 2.0642 mL
60 mM 0.0688 mL 0.3440 mL 0.6881 mL 1.7201 mL
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Moracin M Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Moracin M56317-21-6Phosphodiesterase (PDE)Endogenous MetaboliteNF-κBAutophagyNuclear factor-κBNuclear factor-kappaBA549Airway inflammationInhibitorinhibitorinhibit

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