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  4. MreB Perturbing Compound A22 hydrochloride

MreB Perturbing Compound A22 hydrochloride  (Synonyms: A22 hydrochloride)

Cat. No.: HY-118773 Purity: 99.97%
COA
SDS
Handling Instructions Technical Support

MreB Perturbing Compound A22 hydrochloride (A22 hydrochloride) is a reversible MreB inhibitor and antimicrobial agent that exhibits bacteriostatic activity against Escherichia coli, with low activity against Gram-positive bacteria. MreB Perturbing Compound A22 hydrochloride rapidly and reversally delocalizes MreB from the helical/circular structures in cells, impairs MreB function, and reduces the binding of MreB to the chromosomal region proximal to the replication origin. MreB Perturbing Compound A22 hydrochloride alters the cell morphology of caulobacters to round/lemon-shaped, slows caulobacter growth, blocks their cell division, and induces the formation of spherical morphology, asymmetric division, and anucleate cells in Escherichia coli. MreB Perturbing Compound A22 hydrochloride is used for studies related to bacterial rod-shaped morphology determination, chromosome segregation, and cell division mechanisms.

For research use only. We do not sell to patients.

MreB Perturbing Compound A22 hydrochloride

MreB Perturbing Compound A22 hydrochloride Chemical Structure

CAS No. : 22816-60-0

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of MreB Perturbing Compound A22 hydrochloride:

MreB Perturbing Compound A22 hydrochloride Related Antibodies

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  • Purity & Documentation

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Description

MreB Perturbing Compound A22 hydrochloride (A22 hydrochloride) is a reversible MreB inhibitor and antimicrobial agent that exhibits bacteriostatic activity against Escherichia coli, with low activity against Gram-positive bacteria. MreB Perturbing Compound A22 hydrochloride rapidly and reversally delocalizes MreB from the helical/circular structures in cells, impairs MreB function, and reduces the binding of MreB to the chromosomal region proximal to the replication origin. MreB Perturbing Compound A22 hydrochloride alters the cell morphology of caulobacters to round/lemon-shaped, slows caulobacter growth, blocks their cell division, and induces the formation of spherical morphology, asymmetric division, and anucleate cells in Escherichia coli. MreB Perturbing Compound A22 hydrochloride is used for studies related to bacterial rod-shaped morphology determination, chromosome segregation, and cell division mechanisms[1][2].

Cellular Effect
Cell Line Type Value Description References
A-431 IC50
7.3 μM
Compound: 3s
Inhibition of indoleamine-2,3-dioxygenase in human A431 cells assessed as inhibition of IFN-gamma-stimulated kynurenine production
Inhibition of indoleamine-2,3-dioxygenase in human A431 cells assessed as inhibition of IFN-gamma-stimulated kynurenine production
[PMID: 20688518]
In Vitro

MreB Perturbing Compound A22 (0.1-100 μg/mL; up to 6 h) hydrochloride slows Caulobacter crescentus growth in a dose-dependent manner and causes a bacteriostatic block in cell division at concentrations of 10 μg/mL and higher[1].
MreB Perturbing Compound A22 (0.1-10 μg/mL; 0-8 h) hydrochloride induces dose-dependent morphological changes in Caulobacter crescentus cells, converting crescent-shaped cells to rounded, lemon-shaped cells at 0.1, 1, and 10 μg/mL after 6 h of treatment[1].
MreB Perturbing Compound A22 (10 mg/mL; 24 h) hydrochloride induces anucleate cell production and growth inhibition in Escherichia coli K-12 strain SH3210 as detected by the anucleate cell blue assay[2].
MreB Perturbing Compound A22 hydrochloride shows antibacterial activity with an MIC of 3.1 μg/mL for Escherichia coli, 12.5 μg/mL for Staphylococcus aureus, and >100 μg/mL for Bacillus subtilis[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

MreB Perturbing Compound A22 hydrochloride Related Antibodies

Cell Proliferation Assay[2]

Cell Line: Escherichia coli
Concentration: 10 μg/mL (A22 alone; A22 + 10 μg/mL cephalexin)
Incubation Time: 0-6 h
Result: Slowed cell growth but did not induce lysis when used alone.
Slowed growth further but also did not induce cell lysis when used in combination with 10 μg/mL cephalexin.
Molecular Weight

271.59

Formula

C8H9Cl3N2S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

ClC1=CC(CSC(N)=N)=CC=C1Cl.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 20 mg/mL (73.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : ≥ 20 mg/mL (73.64 mM)

Ethanol : ≥ 1 mg/mL (3.68 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.6820 mL 18.4101 mL 36.8202 mL
5 mM 0.7364 mL 3.6820 mL 7.3640 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.97%

SDS (394 KB)

COA (247 KB) HNMR (255 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO / DMF 1 mM 3.6820 mL 18.4101 mL 36.8202 mL 92.0505 mL
DMSO / DMF 5 mM 0.7364 mL 3.6820 mL 7.3640 mL 18.4101 mL
10 mM 0.3682 mL 1.8410 mL 3.6820 mL 9.2051 mL
15 mM 0.2455 mL 1.2273 mL 2.4547 mL 6.1367 mL
20 mM 0.1841 mL 0.9205 mL 1.8410 mL 4.6025 mL
25 mM 0.1473 mL 0.7364 mL 1.4728 mL 3.6820 mL
30 mM 0.1227 mL 0.6137 mL 1.2273 mL 3.0684 mL
40 mM 0.0921 mL 0.4603 mL 0.9205 mL 2.3013 mL
50 mM 0.0736 mL 0.3682 mL 0.7364 mL 1.8410 mL
60 mM 0.0614 mL 0.3068 mL 0.6137 mL 1.5342 mL
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MreB Perturbing Compound A22 hydrochloride Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MreB Perturbing Compound A2222816-60-0A22MreB Perturbing Compound A 22MreB Perturbing Compound A-22A 22A-22BacterialStaphylococcus aureusBacillus subtilisGram-positive bacteriaCaulobacterorigin-distal lociorigin-proximal chromosomal regionsMreBEscherichia coliorigin-proximal chromosomal lociCaulobacter crescentusInhibitorinhibitorinhibit

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