N,S-Diacetylcysteamine

N,S-Diacetylcysteamine (compound 6) is efficiently used by purified PatB as an acetyl donor to modify the peptidoglycan mimic NAG, with optimal catalytic efficiency (k_cat/K_m = 1103 M^-1s^-1) at pH 8.5^[1].
N,S-Diacetylcysteamine combined with PatB protects isolated peptidoglycan from Bacillus subtilis ΔOat, Escherichia coli, Pseudomonas putida, and Vibrio parahemolyticus against lysozyme degradation in vitro^[1].
N,S-Diacetylcysteamine combined with PatB results in minimal fluorescent labeling of peptidoglycan in intact Bacillus subtilis ΔOat and wild-type cells, indicating low efficiency for whole-cell PG modification relative to alkynyl SNAc analogs^[1].
N,S-Diacetylcysteamine acts as an acetyl-group donor for pigeon liver arylamine acetylase, with a K_m of 300 × 10^-4 M (with 5.8 × 10^-3 M INH), and excessive concentrations inhibit the enzyme^[2].
N,S-Diacetylcysteamine (0.001-0.50 M) undergoes carbon-carbon condensation with the carbanion forms of malononitrile (k₂ = 88 M^-1 min^-1), N-acetyl-S-cyanoacetylcysteamine (k₂ = 0.084 M^-1 min^-1), and ethyl cyanoacetate (k₂ = 3.0 M^-1 min^-1) in aqueous solution at 25°C and 1 M ionic strength, forming acetylated carbanion products and N-acetylcysteamine^[4].
N,S-Diacetylcysteamine (1.0×10^-4 M; up to 200 minutes) has acetyl transfer to ethyl cyanoacetate catalyzed by imidazole in aqueous solution at 25°C and 1 M ionic strength, increasing the reaction rate by ~10³-fold at 1 M imidazole and pH 7, with product yields decreasing as imidazole concentration increases^[4].
N,S-diacetylcysteamine (0.17 mM) undergoes hydroxide-catalyzed hydrolysis in aqueous solution at 25°C and 1 M ionic strength with a second-order rate constant of 9.1 M^-1 min^-1[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.