Cat. No.: HY-B0329

Isoniazid

(Synonyms: INH; Isonicotinic acid hydrazide; Isonicotinic hydrazide)

15 Cited Publications

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Based on 15 publication(s) in Google Scholar

Isoniazid (INH) is a proagent and must be activated by a bacterial catalase-peroxidase enzyme KatG. Isoniazid is bactericidal to rapidly dividing mycobacteria and has anti-tuberculostatic activity.

For research use only. We do not sell to patients.

Purity: 99.95%
CAS No.: 54-85-3
Formula: C₆H₇N₃O
Molecular Weight:137.14

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isoniazid Chemical Structure

CAS No.: 54-85-3

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

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- In Vitro
Chemical Information
Publications (15)
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Bioz

Biological Activity

Description

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>450 μM Compound: INH	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 24292339]
A549	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against human A549 assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against human A549 assessed as inhibition of cell proliferation by MTT assay	[PMID: 25771110]
A549	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against human A549 cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 27214509]
A549	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against Homo sapiens (human) A549 cells after 72 hr by MTT assay Cytotoxicity against Homo sapiens (human) A549 cells after 72 hr by MTT assay	10.1007/s00044-012-0112-0
BMDM	CC₅₀	100 μg/mL Compound: Isoniazid	Cytotoxicity against Swiss mouse BMDM assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against Swiss mouse BMDM assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 28712709]
BMDM	CC₅₀	>20 μg/mL Compound: INH	Cytotoxicity against mouse BMDM cells Cytotoxicity against mouse BMDM cells	[PMID: 35307568]
CHO	IC₅₀	>100 μM Compound: INH	Cytotoxicity against CHO cells Cytotoxicity against CHO cells	[PMID: 29373270]
CHO	CC₅₀	256 μM Compound: INH	Cytotoxicity against CHO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity against CHO cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31525660]
HCT-8	EC₅₀	>100 μM Compound: INH	Toxicity against human HCT8 cells after 72 hrs by XTT assay Toxicity against human HCT8 cells after 72 hrs by XTT assay	[PMID: 20060303]
HepG2	IC₅₀	>1 mM Compound: INH	Cytotoxicity against human HepG2 cells measured after overnight incubation by MTT assay Cytotoxicity against human HepG2 cells measured after overnight incubation by MTT assay	[PMID: 21855181]
HepG2	IC₅₀	>1 mM Compound: INH, Isoniazid	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 22018878]
HepG2	IC₅₀	>250 μM Compound: INH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 24 hrs by MTS assay	[PMID: 24953953]
HepG2	IC₅₀	>250 μM Compound: INH	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 25593095]
HepG2	IC₅₀	>5000 μM Compound: INH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTS assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability by MTS assay	[PMID: 29679902]
HepG2	IC₅₀	>250 μM Compound: INH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after overnight incubation by MTT assay	[PMID: 31401536]
HepG2	IC₅₀	>1000 μM Compound: INH	Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability Cytotoxicity against human HepG2 cells assessed as inhibition of cell viability	[PMID: 37423128]
HepG2	IC₅₀	>100 μM Compound: INH	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability Cytotoxicity against human HepG2 cells assessed as reduction in cell viability	[PMID: 38232131]
J774.1	IC₅₀	>400 μM Compound: V, Isnz	Cytotoxicity against mouse J774.1 cells assessed as cell viability after 48 hrs by MTT assay Cytotoxicity against mouse J774.1 cells assessed as cell viability after 48 hrs by MTT assay	[PMID: 23202852]
J774.A1	IC₅₀	>100 μM Compound: INH	Cytotoxicity against mouse J774A1 cells assessed as cell viability after 24 hrs by Trypan blue exclusion assay Cytotoxicity against mouse J774A1 cells assessed as cell viability after 24 hrs by Trypan blue exclusion assay	[PMID: 24611928]
J774.A1	IC₅₀	>64 μg/mL Compound: INH	Cytotoxicity against mouse J774.A1 cells after 48 hrs by MTT assay Cytotoxicity against mouse J774.A1 cells after 48 hrs by MTT assay	[PMID: 36150342]
Macrophage	IC₅₀	>100 μM Compound: INH	Cytotoxicity against mouse bone marrow derived macrophage assessed as growth inhibition after 48 hrs by MTS assay Cytotoxicity against mouse bone marrow derived macrophage assessed as growth inhibition after 48 hrs by MTS assay	[PMID: 23454512]
MRC5	CC₅₀	>10 μg/mL Compound: INH	Cytotoxicity against human MRC5 cells after 24 hrs Cytotoxicity against human MRC5 cells after 24 hrs	[PMID: 26254828]
MT4	CC₅₀	>100 μM Compound: Isoniazid	Cytotoxicity of compound against mock-infected MT-4 cells was evaluated by MTT assay Cytotoxicity of compound against mock-infected MT-4 cells was evaluated by MTT assay	[PMID: 15006398]
PBMC	IC₅₀	>100 μg/mL Compound: isoniazid	Toxicity against adherent human PBMC Toxicity against adherent human PBMC	[PMID: 17562368]
PBMC	IC₅₀	87.08 μg/mL Compound: isoniazid	Toxicity against non-adherent human PBMC Toxicity against non-adherent human PBMC	[PMID: 17562368]
PBMC	IC₅₀	4.67 mM Compound: INH	Cytotoxicity against human PBMC measured after overnight incubation by MTT assay Cytotoxicity against human PBMC measured after overnight incubation by MTT assay	[PMID: 21855181]
PBMC	IC₅₀	4.67 mM Compound: INH, Isoniazid	Cytotoxicity against human PBMC by MTT assay Cytotoxicity against human PBMC by MTT assay	[PMID: 22018878]
PBMC	IC₅₀	169 μg/mL Compound: INH	Cytotoxicity against PBMC (unknown origin) assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against PBMC (unknown origin) assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 25908515]
PBMC	IC₅₀	71.68 μg/mL Compound: INZ	Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay Cytotoxicity against human PBMC assessed as reduction in cell growth after 72 hrs by MTT assay	[PMID: 32977201]
Peritoneal macrophage	IC₅₀	>100 μg/mL Compound: 3	Cytotoxicity against C57BL/6 mouse peritoneal macrophages assessed as cell viability by lactate dehydrogenase release assay Cytotoxicity against C57BL/6 mouse peritoneal macrophages assessed as cell viability by lactate dehydrogenase release assay	[PMID: 26725953]
RAW264.7	IC₅₀	>400 μg/mL Compound: INH	Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 25908515]
RAW264.7	GI₅₀	3000 μg/mL Compound: INH	Cytotoxicity against mouse RAW264.7 cells Cytotoxicity against mouse RAW264.7 cells	[PMID: 25921267]
RAW264.7	CC₅₀	>150 μM Compound: INH	Cytotoxicity against mouse RAW264.7 cells Cytotoxicity against mouse RAW264.7 cells	[PMID: 33071055]
RAW264.7	GI₅₀	21900 μM Compound: INH	Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay Cytotoxicity against mouse RAW264.7 cells assessed as growth inhibition after 48 hrs by trypan blue assay	10.1039/C3MD00251A
Sf21	IC₅₀	>1000 μM Compound: Isoniazid	Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of human BSEP expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
Sf21	IC₅₀	>1000 μM Compound: Isoniazid	Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake Inhibition of Sprague-Dawley rat Bsep expressed in plasma membrane vesicles of Sf21 cells assessed as inhibition of ATP-dependent [3H]taurocholate uptake	[PMID: 21965623]
THP-1	IC₅₀	>3649 μM Compound: INH	Cytotoxicity against human THP1 cells after 72 hrs by MTT assay Cytotoxicity against human THP1 cells after 72 hrs by MTT assay	[PMID: 21309577]
THP-1	IC₅₀	0.0024 μg/mL Compound: Isoniazid	Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive active phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive active phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay	[PMID: 26299346]
THP-1	IC₅₀	0.0024 μg/mL Compound: Isoniazid	Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive dormant phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay Anti-tubercular activity against rifampicin, isoniazid, ethambutol and pyrazinamide-sensitive dormant phage of Mycobacterium tuberculosis H37Ra ATCC 25177 infected in human THP1 cells by neutral red assay	[PMID: 26299346]
THP-1	IC₅₀	547 μM Compound: INH	Cytotoxicity against human THP1 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as growth inhibition after 24 to 48 hrs by MTT assay	[PMID: 29529504]
THP-1	IC₅₀	>25 μg/mL Compound: INH	Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human THP1 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 31557610]
THP-1	IC₅₀	100 μM Compound: INH	Cytotoxicity against human THP-1 cells assessed as cell viability measured after 96 hrs by MTT assay Cytotoxicity against human THP-1 cells assessed as cell viability measured after 96 hrs by MTT assay	[PMID: 34242760]
THP-1	CC₅₀	>64 μg/mL Compound: INH	Cytotoxicity against human THP-1 cells incubated for 48 hrs by MTS/PMS assay Cytotoxicity against human THP-1 cells incubated for 48 hrs by MTS/PMS assay	[PMID: 37300914]
Vero	IC₅₀	>1000 μg/mL Compound: Isoniazid	Compound was tested for its cytotoxicity on Vero cells Compound was tested for its cytotoxicity on Vero cells	[PMID: 10762047]
Vero	IC₅₀	>1000 μg/mL Compound: Isoniazid	In vitro cytotoxicity against VERO cell line In vitro cytotoxicity against VERO cell line	[PMID: 12139444]
Vero	IC₅₀	>7000 μM Compound: Isoniazid	In vitro cytotoxicity against VERO cell line In vitro cytotoxicity against VERO cell line	[PMID: 12139444]
Vero	IC₅₀	>454.7 μM Compound: INH	Inhibition of mammalian Vero cell viability at 6.25 and 62.5 ug/ml by MTT assay Inhibition of mammalian Vero cell viability at 6.25 and 62.5 ug/ml by MTT assay	[PMID: 16303302]
Vero	IC₅₀	>62.5 μg/mL Compound: Ison	Cytotoxicity against Vero cell line Cytotoxicity against Vero cell line	[PMID: 16759086]
Vero	IC₅₀	>455.77 μM Compound: isoniazid	Cytotoxicity against Vero cells after 72 hrs by MTT assay Cytotoxicity against Vero cells after 72 hrs by MTT assay	[PMID: 17276683]
Vero	IC₅₀	39.8 μM Compound: isoniazid	Cytotoxicity against Vero cells Cytotoxicity against Vero cells	[PMID: 17562368]
Vero	IC₅₀	>200 μg/mL Compound: isoniazid	Cytotoxicity against Vero cells after 48 hrs by XTT method Cytotoxicity against Vero cells after 48 hrs by XTT method	[PMID: 17888665]
Vero	IC₅₀	>455.8 μM Compound: INH	Cytotoxicity against Vero cells after 72 hrs by MTT assay Cytotoxicity against Vero cells after 72 hrs by MTT assay	[PMID: 17960928]
Vero	IC₅₀	>455.8 μM Compound: INH, isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 18068979]
Vero	IC₅₀	>455.8 μM Compound: isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 18078756]
Vero	IC₅₀	>455.8 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 18304818]
Vero	CC₅₀	>455.8 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 18502542]
Vero	IC₅₀	>455.8 μM Compound: isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19131245]
Vero	IC₅₀	>500 μg/mL Compound: Isoniazid	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 19232450]
Vero	IC₅₀	>128 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer assay Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer assay	[PMID: 19271749]
Vero	IC₅₀	128 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs Cytotoxicity against african green monkey Vero cells after 72 hrs	[PMID: 19757815]
Vero	IC₅₀	62.5 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19879757]
Vero	IC₅₀	>455.8 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 19942326]
Vero	IC₅₀	>62.5 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20056418]
Vero	CC₅₀	5833.5 μM Compound: Isoniazid	Cytotoxicity against african green monkey Vero C1008 cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero C1008 cells after 72 hrs by MTT assay	[PMID: 20138519]
Vero	IC₅₀	>130.5 μM Compound: Isoniazid	Cytotoxicity against african green monkey Vero after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero after 72 hrs by MTT assay	[PMID: 20576327]
Vero	CC₅₀	>455.73 μM Compound: Isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 20615698]
Vero	CC₅₀	>500 μM Compound: INH	Toxicity in African green monkey Vero cells Toxicity in African green monkey Vero cells	[PMID: 20850299]
Vero	IC₅₀	>100 μM Compound: INH	Cytotoxicity against african green monkey Vero cells assessed as cell growth after 48 hrs by hemocytometry Cytotoxicity against african green monkey Vero cells assessed as cell growth after 48 hrs by hemocytometry	[PMID: 20961671]
Vero	CC₅₀	6282.8 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assat Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assat	[PMID: 21146257]
Vero	CC₅₀	>455.73 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21320779]
Vero	CC₅₀	>500 μM Compound: INH, Isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer Glo luminescent cell viability assay Cytotoxicity against african green monkey Vero cells after 72 hrs by Cell titer Glo luminescent cell viability assay	[PMID: 21570839]
Vero	CC₅₀	>455.73 μM Compound: INH	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21840711]
Vero	CC₅₀	1459.85 μM Compound: 1NH	Cytotoxicity against african green monkey Vero C1008 cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero C1008 cells after 72 hrs by MTT assay	[PMID: 21917452]
Vero	IC₅₀	>456 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 21978838]
Vero	IC₅₀	>128 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiter assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiter assay	[PMID: 22391032]
Vero	CC₅₀	>50 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay Cytotoxicity against african green monkey Vero cells after 72 hrs by CellTiterGlo assay	[PMID: 22691154]
Vero	IC₅₀	>62.5 μg/mL Compound: Isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 22749825]
Vero	IC₅₀	>62.5 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23122863]
Vero	IC₅₀	>62.5 μg/mL Compound: Isoniazid	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23352268]
Vero	IC₅₀	>100 μM Compound: INH	Cytotoxicity against african green monkey Vero cells assessed as growth inhibition after 24 hrs by MTS assay Cytotoxicity against african green monkey Vero cells assessed as growth inhibition after 24 hrs by MTS assay	[PMID: 23454512]
Vero	IC₅₀	>100 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay	[PMID: 23886691]
Vero	IC₅₀	>128 μM Compound: INH	Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay Cytotoxicity against african green monkey Vero cells by tetrazolium dye reduction assay	[PMID: 24084159]
Vero	IC₅₀	>100 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay Cytotoxicity against african green monkey Vero cells after 72 hrs by MTS-PMS assay	[PMID: 24087924]
Vero	CC₅₀	1470 μM Compound: INH	Cytotoxicity against African green monkey Vero C1008 cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero C1008 cells after 72 hrs by MTT assay	[PMID: 24095091]
Vero	IC₅₀	>450 μM Compound: INH	Cytotoxicity against african green monkey Vero cells by MTT assay Cytotoxicity against african green monkey Vero cells by MTT assay	[PMID: 24292339]
Vero	IC₅₀	>100 μM Compound: Isoniazid	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 24314672]
Vero	IC₅₀	>1000 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 24582983]
Vero	IC₅₀	>62.5 μg/mL Compound: Isoniazid	Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24721315]
Vero	IC₅₀	>200 μM Compound: INH	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 24836065]
Vero	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation by MTT assay Cytotoxicity against African green monkey Vero cells assessed as inhibition of cell proliferation by MTT assay	[PMID: 25771110]
Vero	CC₅₀	>7000 μM Compound: INH	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 27241693]
Vero	IC₅₀	>50 μM Compound: 51	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by tetrazolium dye assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by tetrazolium dye assay	[PMID: 28094223]
Vero	IC₅₀	>6.25 μg/mL Compound: Isoniazid	Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 28552337]
Vero	IC₅₀	>256 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 28574259]
Vero	CC₅₀	125 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28646656]
Vero	CC₅₀	125 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 28720502]
Vero	CC₅₀	128 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 29227930]
Vero	CC₅₀	32 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 29407980]
Vero	CC₅₀	128 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30025351]
Vero	IC₅₀	>200 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30145051]
Vero	CC₅₀	256 μM Compound: INH	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 30296686]
Vero	CC₅₀	256 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 30690301]
Vero	CC₅₀	256 μM Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay	[PMID: 30992202]
Vero	CC₅₀	>7300 μM Compound: INH	Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay Cytotoxicity in African green monkey Vero cells assessed as reduction in cell viability incubated for 72 hrs by MTT assay	[PMID: 31350124]
Vero	CC₅₀	128 μg/mL Compound: Isoniazid	Cytotoxicity against African green monkey Vero cells incubated for 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells incubated for 72 hrs by MTT assay	[PMID: 31352245]
Vero	CC₅₀	64 μg/mL Compound: Isoniazid	Cytotoxicity in African green monkey Vero cells Cytotoxicity in African green monkey Vero cells	[PMID: 31358466]
Vero	IC₅₀	>50 μM Compound: INH	Cytotoxicity against African green monkey Vero cells Cytotoxicity against African green monkey Vero cells	[PMID: 32031795]
Vero	CC₅₀	>50 μM Compound: INH	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33010635]
Vero	IC₅₀	>455.69 μM Compound: Isoniazid	Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	[PMID: 33482292]
Vero	IC₅₀	>64 μg/mL Compound: INH	Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 48 hrs by MTT assay	[PMID: 36150342]
Vero	IC₅₀	>64 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay Cytotoxicity against african green monkey Vero cells after 48 hrs by MTT assay	[PMID: 37348296]
Vero	IC₅₀	>100 μg/mL Compound: INH	Cytotoxicity against african green monkey Vero cells incubated for 48 hrs by CCK8 assay Cytotoxicity against african green monkey Vero cells incubated for 48 hrs by CCK8 assay	[PMID: 39166949]
Vero	IC₅₀	>128 μM Compound: INH	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells	10.1007/s00044-007-9073-0
Vero	CC₅₀	>455.8 μM Compound: Isoniazid	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as cell viability after 72 hr by MTT assay	10.1007/s00044-011-9638-9
Vero	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr by MTT assay	10.1007/s00044-011-9646-9
Vero	CC₅₀	199 μg/mL Compound: Isoniazid	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero C1008 cells assessed as cell viability after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero C1008 cells assessed as cell viability after 72 hr by MTT assay	10.1007/s00044-012-0043-9
Vero	IC₅₀	>12 μg/mL Compound: INH	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as inhibition of cell viability after 96 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells assessed as inhibition of cell viability after 96 hr by MTT assay	10.1007/s00044-012-0074-2
Vero	IC₅₀	>450 μM Compound: Isoniazid	Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr by MTT assay Cytotoxicity against Chlorocebus aethiops (African green monkey) Vero cells after 72 hr by MTT assay	10.1007/s00044-012-0112-0
WI-38	CC₅₀	>50 μM Compound: Isoniazid	Cytotoxicity against human WI-38 cells assessed as reduction in cell growth Cytotoxicity against human WI-38 cells assessed as reduction in cell growth	[PMID: 38492608]

In Vitro

Isoniazid (INH) is a prodrug and must be activated by a bacterial catalase-peroxidase enzyme that in M. tuberculosis is called KatG^[1].
KatG couples the isonicotinic acyl with NADH to form isonicotinic acyl-NADH complex. This complex binds tightly to the enoyl-acyl carrier protein reductase known as InhA, thereby blocking the natural enoyl-AcpM substrate and the action of fatty acid synthase. This process inhibits the synthesis of mycolic acid, required for the mycobacterial cell wall. A range of radicals are produced by KatG activation of isoniazid, including nitric oxide, which has also been shown to be important in the action of another antimycobacterial prodrug PA-824^[2]^[3].
Isoniazid is bactericidal to rapidly dividing mycobacteria but is bacteriostatic if the mycobacteria are slow-growing^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 54-85-3
Appearance Solid
Molecular Weight 137.14
Formula C₆H₇N₃O
Color White to off-white
SMILES

O=C(C1=CC=NC=C1)NN
Synonyms

INH; Isonicotinic acid hydrazide; Isonicotinic hydrazide
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (15)

All Cell Proliferation/Viability AssayCell Imaging/StainingBio/Physico-chemical Assay

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Journal Impact Factor

Nat Commun

Fundamental role of spatial positioning of Mycobacterium tuberculosis in mycobacterial survival in macrophages. [Abstract]

2025 Oct 23;16(1):9368. PMID: 41130960
Commun Biol

The acetyltransferase CysE modulates virulence and drug resistance of Mycobacterium tuberculosis by interfering with oxidative stress responses. [Abstract]

2025 Oct 6;8(1):1425. PMID: 41053365
Front Bioeng Biotechnol

Application of 3D Hepatic Plate-Like Liver Model for Statin-Induced Hepatotoxicity Evaluation. [Abstract]

2022 Mar 17;10:826093. PMID: 35372314

Isoniazid purchased from MedChemExpress. Usage Cited in: Front Bioeng Biotechnol. 2022 Mar 17;10:826093. [Abstract]

Cytotoxicity analysis of L-02 cells in the four co-culture systems with treatment of isoniazid (INH)) (200, 400, 600, 800, 1000 μM).

Isoniazid purchased from MedChemExpress. Usage Cited in: Front Bioeng Biotechnol. 2022 Mar 17;10:826093. [Abstract]

The 3D-P co-culture system treated with Isoniazid (INH) showed an increase in the AFI of CellROX in a dose-dependent manner.

Isoniazid purchased from MedChemExpress. Usage Cited in: Front Bioeng Biotechnol. 2022 Mar 17;10:826093. [Abstract]

Assessments of ALT, AST, and LDH levels in L-02 cells at 2D and 3D-P co-culture systems with a single treatment of Isoniazid (INH) (200, 400, 600, 800, 1000, 1200 μM, 24 h).
Antimicrob Agents Chemother

Incorporation of macrophage immune stresses into an intracellular assay of drug tolerance in Mycobacterium tuberculosis. [Abstract]

2025 Sep 11:e0079525. PMID: 40934365
Antimicrob Agents Chemother

An enoyl-ACP reductase inhibitor, NITD-916, expresses anti- Mycobacterium abscessus activity. [Abstract]

2025 May 23:e0024925. PMID: 40407337
mLife

Identification of a phenyl ester covalent inhibitor of caseinolytic protease and analysis of the ClpP1P2 inhibition in mycobacteria. [Abstract]

2025 Apr 15;4(2):155-168. PMID: 40313980
Sci Rep

Identification and clustering analysis of drug-responsive temporally varying genes through high-frequency longitudinal RNA sequencing. [Abstract]

2026 Mar 19. PMID: 41857104
ACS Chem Biol

Investigate Natural Product Indolmycin and the Synthetically Improved Analogue Toward Antimycobacterial Agents. [Abstract]

2022 Jan 21;17(1):39-53. PMID: 34908399
Biotechnol Bioeng

An integrated biomimetic array chip for establishment of collagen-based 3D primary human hepatocyte model for prediction of clinical drug-induced liver injury. [Abstract]

2021 Dec;118(12):4687-4698. PMID: 34478150
J Biochem Mol Toxicol

Network Toxicology and Molecular Docking Analysis of Antituberculosis Drug-Induced Hepatotoxicity. [Abstract]

2025 Nov;39(11):e70602. PMID: 41215592
bioRxiv

Ubiquitylation by the GID/CTLH complex regulates the metabolic and innate immune response of macrophages to infection by Mycobacterium tuberculosis. [Abstract]

2026 Apr 27:2026.04.24.720540. PMID: 42094500
bioRxiv

2025 Nov 11.
SSRN

2025 May 7.
Res Sq

Proapoptotic Bcl-2 inhibitor as host directed therapy for pulmonary tuberculosis. [Abstract]

2024 Sep 2:rs.3.rs-4926508. PMID: 39281866
University of Manitoba

2022 Jan.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (364.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 33.33 mg/mL (243.04 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

7.2918 mL

36.4591 mL

72.9182 mL

5 mM

1.4584 mL

7.2918 mL

14.5836 mL

10 mM

0.7292 mL

3.6459 mL

7.2918 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (36.46 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (36.46 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (36.46 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 150 mg/mL (1093.77 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (598 KB)
- English - EN (598 KB)
- Français - FR (598 KB)
- Deutsch - DE (598 KB)
- Norwegian - NO (598 KB)
- Español - ES (598 KB)
- Swedish - SV (598 KB)
- Italian - IT (598 KB)
- Korean - KR (598 KB)
- Portuguese - PT (598 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Suarez, J., et al., An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG). J Biol Chem, 2009. 284(11): p. 7017-29. [Content Brief]

[2]. Timmins, G.S., et al., Nitric oxide generated from isoniazid activation by KatG: source of nitric oxide and activity against Mycobacterium tuberculosis. Antimicrob Agents Chemother, 2004. 48(8): p. 3006-9. [Content Brief]

[3]. Singh, R., et al., PA-824 kills nonreplicating Mycobacterium tuberculosis by intracellular NO release. Science, 2008. 322(5906): p. 1392-5. [Content Brief]

[4]. Ahmad, Z., et al., Biphasic kill curve of isoniazid reveals the presence of drug-tolerant, not drug-resistant, Mycobacterium tuberculosis in the guinea pig. J Infect Dis, 2009. 200(7): p. 1136-43. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	7.2918 mL	36.4591 mL	72.9182 mL	182.2955 mL
	5 mM	1.4584 mL	7.2918 mL	14.5836 mL	36.4591 mL
	10 mM	0.7292 mL	3.6459 mL	7.2918 mL	18.2295 mL
	15 mM	0.4861 mL	2.4306 mL	4.8612 mL	12.1530 mL
	20 mM	0.3646 mL	1.8230 mL	3.6459 mL	9.1148 mL
	25 mM	0.2917 mL	1.4584 mL	2.9167 mL	7.2918 mL
	30 mM	0.2431 mL	1.2153 mL	2.4306 mL	6.0765 mL
	40 mM	0.1823 mL	0.9115 mL	1.8230 mL	4.5574 mL
	50 mM	0.1458 mL	0.7292 mL	1.4584 mL	3.6459 mL
	60 mM	0.1215 mL	0.6077 mL	1.2153 mL	3.0383 mL
	80 mM	0.0911 mL	0.4557 mL	0.9115 mL	2.2787 mL
	100 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8230 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Isoniazid

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Publications Citing Use of MedChemExpress (MCE) Isoniazid

All Antibiotic Isoforms

Biological Activity

Chemical Information

Publications (15)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Isoniazid Related Classifications

Cat. No.: HY-B0329