Nonaethylene glycol monododecyl ether
Based on 1 Customer Validation
Nonaethylene glycol monododecyl ether (Nonaoxyethylene monododecyl ether) is a nonionic surfactant and polyethylene glycol (PEG) detergent that can be used to form initial coalesced O/W emulsion droplets, as well as for protein separation and purification.
For research use only. We do not sell to patients.
- Purity: 99.70%
- CAS No.: 3055-99-0
- Formula: C30H62O10
- Molecular Weight:582.81
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Storage:Pure form -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-N0324
Cholic acid
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HY-13771
Ursodeoxycholic acid
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HY-108294R
Nonaethylene glycol monododecyl ether (Standard)
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HY-76847
Chenodeoxycholic Acid
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HY-D1005
Poloxamer 407 (F127)
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HY-D1005A
Poloxamer 188
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HY-N0324R
Cholic acid (Standard)
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HY-D1005H
Poloxamer 188 (F68)
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HY-D1005A6
Poloxamer 124 (L44)
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HY-13771R
Ursodeoxycholic acid (Standard)
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HY-D1005A22
Poloxamer 338 (F108)
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HY-76847R
Chenodeoxycholic Acid (Standard)
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | CC50 |
10.3 μM
Compound: POLIDOCANOL
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Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
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10.21203/rs.3.rs-23951/v1 |
| Caco-2 | IC50 |
0.22 μM
Compound: POLIDOCANOL
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Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
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10.21203/rs.3.rs-23951/v1 |
Examination of a series of non-ionic PEG detergents with several long-chain E-PDSs from different organisms reveals that in vitro incubations with Nonaethylene glycol monododecyl ether typically gave chain lengths that corresponded to those of the isoprenoid moieties in respiratory quinones synthesized in vivo[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3055-99-0
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Appearance Oil
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Molecular Weight 582.81
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Formula C30H62O10
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Color White to off-white
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SMILES
CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO
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Synonyms
Nonaoxyethylene monododecyl ether
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Pure form -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (343.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Ethanol : 100 mg/mL (171.58 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
[1]. Li C, et al. Oil-in-Water Emulsion Templated and Crystallization-Driven Self-Assembly Formation of Poly(l-lactide)-Polyoxyethylene-Poly(l-lactide) Fibers. Langmuir. 2017 Nov 14;33(45):13060-13067. [Content Brief]
[2]. Pan JJ, et al. Dependence of the product chain-length on detergents for long-chain E-polyprenyl diphosphate synthases. Biochemistry. 2013 Jul 23;52(29):5002-8. [Content Brief]
[3]. Zhang W, et al. Comparison of the different types of surfactants for the effect on activity and structure of soybean peroxidase. Langmuir. 2009 Feb 17;25(4):2363-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol / DMSO | 1 mM | 1.7158 mL | 8.5791 mL | 17.1583 mL | 42.8956 mL |
| 5 mM | 0.3432 mL | 1.7158 mL | 3.4317 mL | 8.5791 mL | |
| 10 mM | 0.1716 mL | 0.8579 mL | 1.7158 mL | 4.2896 mL | |
| 15 mM | 0.1144 mL | 0.5719 mL | 1.1439 mL | 2.8597 mL | |
| 20 mM | 0.0858 mL | 0.4290 mL | 0.8579 mL | 2.1448 mL | |
| 25 mM | 0.0686 mL | 0.3432 mL | 0.6863 mL | 1.7158 mL | |
| 30 mM | 0.0572 mL | 0.2860 mL | 0.5719 mL | 1.4299 mL | |
| 40 mM | 0.0429 mL | 0.2145 mL | 0.4290 mL | 1.0724 mL | |
| 50 mM | 0.0343 mL | 0.1716 mL | 0.3432 mL | 0.8579 mL | |
| 60 mM | 0.0286 mL | 0.1430 mL | 0.2860 mL | 0.7149 mL | |
| 80 mM | 0.0214 mL | 0.1072 mL | 0.2145 mL | 0.5362 mL | |
| 100 mM | 0.0172 mL | 0.0858 mL | 0.1716 mL | 0.4290 mL |
Nonaethylene glycol monododecyl ether Related Classifications
HY-108294 Related Classifications
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