Norathyriol
Based on 3 publication(s) in Google Scholar
Norathyriol (Mangiferitin) is a natural metabolite of Mangifera. Norathyriol inhibits α-glucosidase in a noncompetitive manner with an IC50 of 3.12 μM. Norathyriol inhibits PPARα, PPARβ, and PPARγ with IC50s of 92.8 µM, 102.4 µM, and 153.5 µM, respectively. Antioxidant, anticancer, antimicrobial, anti-inflammatory, anti-bacterial activities.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 3542-72-1
- Formula: C13H8O6
- Molecular Weight:260.20
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Norathyriol
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Biological Activity
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PPARα 92.8 μM (IC50) |
PPARβ 102.4 μM (IC50) |
PPARγ 153.5 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| COLO 320 | IC50 |
23 μM
Compound: 45359
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Antiproliferative activity against human COLO320 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human COLO320 cells after 48 hrs by CCK8 assay
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[PMID: 28376372] |
| HeLa S3 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HeLaS3 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| HepG2 | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human HepG2 cells by MTT assay
Cytotoxicity against human HepG2 cells by MTT assay
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[PMID: 24960143] |
| HepG2 | IC50 |
23.7 μM
Compound: 45359
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Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human HepG2 cells after 48 hrs by CCK8 assay
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[PMID: 28376372] |
| HepG2 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HepG2 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| HT-29 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human HT-29 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| K562 | IC50 |
13.1 μM
Compound: 45359
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Antiproliferative activity against human K562 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human K562 cells after 48 hrs by CCK8 assay
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[PMID: 28376372] |
| KB | IC50 |
>10 μM
Compound: 21
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Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay
Cytotoxicity in human KB cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| L02 | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human HL7702 cells by MTT assay
Cytotoxicity against human HL7702 cells by MTT assay
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[PMID: 24960143] |
| MCF7 | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human MCF7 cells by MTT assay
Cytotoxicity against human MCF7 cells by MTT assay
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[PMID: 24960143] |
| MCF7 | IC50 |
43.2 μM
Compound: 45359
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Antiproliferative activity against human MCF7 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human MCF7 cells after 48 hrs by CCK8 assay
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[PMID: 28376372] |
| MCF7 | IC50 |
>10 μM
Compound: 21
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Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay
Cytotoxicity in human MCF7 cells incubated for 72 hrs by MTT assay
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[PMID: 30978023] |
| MDA-MB-231 | IC50 |
>10 μM
Compound: 18
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Cytotoxicity against human MDA-MB-231 cells by MTT assay
Cytotoxicity against human MDA-MB-231 cells by MTT assay
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[PMID: 24960143] |
| MDA-MB-231 | IC50 |
23.6 μM
Compound: 45359
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Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by CCK8 assay
Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by CCK8 assay
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[PMID: 28376372] |
| RAW264.7 | IC50 |
43 μM
Compound: 18
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma plus LPS-induced NO production
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of IFN-gamma plus LPS-induced NO production
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[PMID: 24960143] |
Norathyriol (1-25 µM) inhibits growth by inducing cell cycle arrest in JB6 P+ cells. Norathyriol inhibits JB6 cell growth by inducing G2-M arrest[3].
Norathyriol suppresses UVB-induced phosphorylation of ERKs, AP-1 and NF-κB activation in JB6 P+ cells[3]
Cell Growth Assay
WB
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Mouse skin epidermal JB6 P+ cells
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Concentration:0, 1, 10, or 25 µM
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Incubation Time:24 or 72 hours
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Result:Inhibited cell growth in a dose- as well as time-dependent manner but does not cause cell death.
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Cell Line:JB6 P+ cells
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Concentration:0, 1, 10, or 25 µM
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Incubation Time:2 hours
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Result:Inhibited UVB-induced phosphorylation of ERKs and p90RSK.
Norathyriol (0.92, 1.85 and 3.7 mg/kg) dose dependently decreased the serum urate levels by 27.0, 33.6 and 37.4%, respectively[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Adult Kunming mice weighing 18-22 g[4]
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Dosage:0.92, 1.85 and 3.7 mg/kg
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Administration:Administered intragastrically; twice daily for five times
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Result:The serum uric acid levels were decreased by 27.0%, 33.6% and 37.4%.
Chemical Information
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CAS No. 3542-72-1
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Appearance Solid
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Molecular Weight 260.20
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Formula C13H8O6
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Color White to light yellow
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SMILES
O=C1C2=C(OC3=C1C=C(O)C(O)=C3)C=C(O)C=C2O
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Synonyms
Mangiferitin
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment. [Abstract]2025 Jan 2;16(1):333. PMID: 39747873 -
Cell Death Dis
CILP1 interacting with YBX1 promotes hypertrophic scar formation by suppressing PPARs transcription. [Abstract]2025 May 9;16(1):371. PMID: 40346063 -
Sci Rep
Polysaccharide from Prinsepia utilis Royle maintains the skin barrier by mediating differentiation, lipid metabolism and tight junction of keratinocyte. [Abstract]2025 Jul 1;15(1):20470. PMID: 40595762
Solvent & Solubility
DMSO : 100 mg/mL (384.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.61 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhi-Long Shi, et al. In Vitro and In Vivo Effects of Norathyriol and Mangiferin on α-Glucosidase. Biochem Res Int. 2017;2017:1206015. [Content Brief]
[2]. Ashley S Wilkinson,et al. Effects of the mango components mangiferin and quercetin and the putative mangiferin metabolite norathyriol on the transactivation of peroxisome proliferator-activated receptor isoforms. J Agric Food Chem. 2008 May 14;56(9):3037-42. [Content Brief]
[3]. Jixia Li, et al. Norathyriol suppresses skin cancers induced by solar ultraviolet radiation by targeting ERK kinases. Cancer Res. 2012 Jan 1;72(1):260-70. [Content Brief]
[4]. Yanfen Niu, et al. Hypouricaemic action of mangiferin results from metabolite norathyriol via inhibiting xanthine oxidase activity. Pharm Biol. 2016 Sep;54(9):1680-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8432 mL | 19.2160 mL | 38.4320 mL | 96.0799 mL |
| 5 mM | 0.7686 mL | 3.8432 mL | 7.6864 mL | 19.2160 mL | |
| 10 mM | 0.3843 mL | 1.9216 mL | 3.8432 mL | 9.6080 mL | |
| 15 mM | 0.2562 mL | 1.2811 mL | 2.5621 mL | 6.4053 mL | |
| 20 mM | 0.1922 mL | 0.9608 mL | 1.9216 mL | 4.8040 mL | |
| 25 mM | 0.1537 mL | 0.7686 mL | 1.5373 mL | 3.8432 mL | |
| 30 mM | 0.1281 mL | 0.6405 mL | 1.2811 mL | 3.2027 mL | |
| 40 mM | 0.0961 mL | 0.4804 mL | 0.9608 mL | 2.4020 mL | |
| 50 mM | 0.0769 mL | 0.3843 mL | 0.7686 mL | 1.9216 mL | |
| 60 mM | 0.0641 mL | 0.3203 mL | 0.6405 mL | 1.6013 mL | |
| 80 mM | 0.0480 mL | 0.2402 mL | 0.4804 mL | 1.2010 mL | |
| 100 mM | 0.0384 mL | 0.1922 mL | 0.3843 mL | 0.9608 mL |
Norathyriol Related Classifications
HY-N1029 Related Classifications
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