O-(4-Nitrobenzyl)hydroxylamine hydrochloride

Cat. No.: HY-W012469 Purity: 99.78%: Data Sheet
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Handling Instructions
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O-(4-Nitrobenzyl)hydroxylamine hydrochloride is a biochemical assay reagent used for the preparation of N-(4-nitrobenzyloxy)-amino acid as substrates for an unambiguous N-hydroxypeptide synthesis.

For research use only. We do not sell to patients.

O-(4-Nitrobenzyl)hydroxylamine hydrochloride Chemical Structure

CAS No. : 2086-26-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
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Description

O-(4-Nitrobenzyl)hydroxylamine hydrochloride is a biochemical assay reagent used for the preparation of N-(4-nitrobenzyloxy)-amino acid as substrates for an unambiguous N-hydroxypeptide synthesis.

Cellular Effect

Cell Line	Type	Value	Description	References
SK-OV-3	IC₅₀	8 μM Compound: 2b.HCl	Inhibition of kynurenine production in human SK-OV-3 cells Inhibition of kynurenine production in human SK-OV-3 cells	[PMID: 38621594]
SK-OV-3	IC₅₀	> 100 μM Compound: 2b.HCl	Cytotoxicity against human SK-OV-3 cells Cytotoxicity against human SK-OV-3 cells	[PMID: 38621594]

Molecular Weight

204.61

Formula

C₇H₉ClN₂O₃

CAS No.

2086-26-2

Appearance

Solid

Color

White to off-white

SMILES

NOCC1=CC=C(C=C1)[N+]([O-])=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (488.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.8873 mL	24.4367 mL	48.8735 mL
5 mM	0.9775 mL	4.8873 mL	9.7747 mL
10 mM	0.4887 mL	2.4437 mL	4.8873 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

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Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.84%

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Data Sheet (242 KB) SDS (394 KB)

COA (246 KB) HNMR (116 KB) RP-HPLC (253 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.8873 mL	24.4367 mL	48.8735 mL	122.1837 mL
	5 mM	0.9775 mL	4.8873 mL	9.7747 mL	24.4367 mL
	10 mM	0.4887 mL	2.4437 mL	4.8873 mL	12.2184 mL
	15 mM	0.3258 mL	1.6291 mL	3.2582 mL	8.1456 mL
	20 mM	0.2444 mL	1.2218 mL	2.4437 mL	6.1092 mL
	25 mM	0.1955 mL	0.9775 mL	1.9549 mL	4.8873 mL
	30 mM	0.1629 mL	0.8146 mL	1.6291 mL	4.0728 mL
	40 mM	0.1222 mL	0.6109 mL	1.2218 mL	3.0546 mL
	50 mM	0.0977 mL	0.4887 mL	0.9775 mL	2.4437 mL
	60 mM	0.0815 mL	0.4073 mL	0.8146 mL	2.0364 mL
	80 mM	0.0611 mL	0.3055 mL	0.6109 mL	1.5273 mL
	100 mM	0.0489 mL	0.2444 mL	0.4887 mL	1.2218 mL