1. Membrane Transporter/Ion Channel
    Neuronal Signaling
  2. GABA Receptor

Aminooxyacetic acid hemihydrochloride (Synonyms: Carboxymethoxylamine Hemihydrochloride)

Cat. No.: HY-107994 Purity: >98.0%
Handling Instructions

Aminooxyacetic acid hemihydrochloride is a malate-aspartate shuttle (MAS) inhibitor which also inhibits the GABA degradating enzyme GABA-T.

For research use only. We do not sell to patients.

Aminooxyacetic acid hemihydrochloride Chemical Structure

Aminooxyacetic acid hemihydrochloride Chemical Structure

CAS No. : 2921-14-4

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 92 In-stock
Estimated Time of Arrival: December 31
1 g USD 84 In-stock
Estimated Time of Arrival: December 31
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Aminooxyacetic acid hemihydrochloride is a malate-aspartate shuttle (MAS) inhibitor which also inhibits the GABA degradating enzyme GABA-T.

IC50 & Target

MAS[1], GABA-T[2]

In Vitro

Aminooxyacetic acid hemihydrochloride (AOAA) dose-dependently decreases the survival of C6 glioma cells. Aminooxyacetic acid hemihydrochloride treatment produces a significant increase in the percentage of the cells arrested in the stage of G0/G1, as well as a significant decrease in the percentage of the cells at S phase and G2/M phase. Aminooxyacetic acid hemihydrochloride treatment leads to an obvious decrease in the number of the cells in the phase of cell division. Aminooxyacetic acid hemihydrochloride significantly increases the percentage of the cells in both early-stage apoptosis and necrosis. Treatment of the cells with 1 mM or 5 mM Aminooxyacetic acid hemihydrochloride leads to decreased levels of aging of the cells[1]. Glutamine-dependent cell lines show greater inhibition of cell growth by Aminooxyacetic acid hemihydrochloride (AOA) compare with cells that are less glutamine dependent[3].

In Vivo

The accumulation of GABA in cerebellum and whole brain is initially very rapid, being significantly increased already 5 min after the injection of Aminooxyacetic acid hemihydrochloride (AOAA). The rapid initial accumulation becomes gradually slower and maximal levels (400 to 600 % of the control levels) are reached 2 to 6 h after Aminooxyacetic acid hemihydrochloride. Still 24 h after Aminooxyacetic acid hemihydrochloride the GABA levels are elevated by about 250%. From 2 to 6 h after Aminooxyacetic acid hemihydrochloride the convulsions are completely blocked. Twenty four hours after Aminooxyacetic acid hemihydrochloride the convulsions are almost identical to the controls[2].

Clinical Trial
Solvent & Solubility
In Vitro: 

DMSO : 42.9 mg/mL (392.50 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 9.1491 mL 45.7457 mL 91.4913 mL
5 mM 1.8298 mL 9.1491 mL 18.2983 mL
10 mM 0.9149 mL 4.5746 mL 9.1491 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[3]

Enzyme activity of aspartate transaminase is measured by a colorimetric assay assessing formation of pyruvate from oxaloacetate, a product of GOT1/2 (also called AST1/2) activity, as described previously. In brief, cells grown in 6-well plates are collected after 6, 24, or 48 hours of Aminooxyacetic acid hemihydrochloride (AOA) treatment and washed with cold PBS, lysed, and supernatant used for analysis[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[3]

Breast cancer cell lines are used in this study. Cells are plated in 96-well plates at 1,500 to 5,000 cells per well in 100 μL media. New medium with varying concentration of Aminooxyacetic acid hemihydrochloride (AOA) is added after 12 hours. The assay is performed after 48 hours[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Female albino rats (150 to 200 g) bred at our department are used. Aminooxyacetic acid hemihydrochloride (AOAA) is injected into a tail vein (2 mL/kg body weight). For this purpose the rat is placed in a plastic tube and the tail warmed in water 42°C[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

109.30

Formula

NH₂OCH₂COOH · ₀.₅HCl

CAS No.

2921-14-4

SMILES

OC(CON)=O.[H]Cl.OC(CON)=O

Storage

4°C, protect from light

Shipping

Room temperature in continental US; may vary elsewhere

Purity: >98.0%

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Product Name:
Aminooxyacetic acid hemihydrochloride
Cat. No.:
HY-107994
Quantity:

Aminooxyacetic acid hemihydrochloride

Cat. No.: HY-107994