Aminooxyacetic acid hemihydrochloride
Based on 25 publication(s) in Google Scholar
Aminooxyacetic acid (Carboxymethoxylamine) hemihydrochloride is a malate-aspartate shuttle (MAS) inhibitor which also inhibits the GABA degradating enzyme GABA-T.
For research use only. We do not sell to patients.
- Purity: 98.0%
- CAS No.: 2921-14-4
- Formula: C2H5NO3.1/2HCl
- Molecular Weight:109.30
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Aminooxyacetic acid hemihydrochloride
More- Signal Transduct Target Ther. 2023 Feb 15;8(1):65. [Abstract]
- Nat Metab. 2022 Feb;4(2):239-253. [Abstract]
- Cancer Res. 2022 Sep 16;82(18):3223-3235. [Abstract]
- Redox Biol. 2024 May:71:103112. [Abstract]
- Theranostics. 2021 Mar 24;11(12):5650-5674. [Abstract]
- Sci Adv. 2025 Aug 29;11(35):eadu6271. [Abstract]
- Sci Adv. 2025 Aug 15;11(33):eadw9241. [Abstract]
- Research (Wash D C). 2025 May 23:8:0703. [Abstract]
- Phytomedicine. 2025 Nov 25:148:157492. [Abstract]
- Cell Death Discov. 2025 Oct 6;11(1):430. [Abstract]
- Phytother Res. 2026 May;40(5):2569-2587. [Abstract]
- ACS Appl Mater Interfaces. 2025 Dec 24;17(51):69180-69195. [Abstract]
- Stem Cell Res Ther. 2025 Oct 31;16(1):603. [Abstract]
- CNS Neurosci Ther. 2023 Mar;29(3):941-956. [Abstract]
- Cell Mol Life Sci. 2024 May 3;81(1):204. [Abstract]
- Mol Nutr Food Res. 2023 Nov;67(22):e2300112. [Abstract]
- ASN Neuro. 2021 Jan-Dec:13:17590914211043087. [Abstract]
- FASEB J. 2026 May 31;40(10):e71888. [Abstract]
- J Virol. 2025 Nov 25;99(11):e0098525. [Abstract]
- Viruses. 2024 Sep 29;16(10):1540. [Abstract]
- Am J Transl Res. 2018 Dec 15;10(12):4247-4257. [Abstract]
- Res Sq. 2026 Mar 5.
- bioRxiv. 2025 July 11.
- Biomed Pharmacother. 2023 Nov:167:115605. [Abstract]
- Comput Biol Med. 2023 Oct:165:107415. [Abstract]
Customer Validation & Images
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Cell Migration/Invasion Assay
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In Vivo Efficacy Study
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WB
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
Biological Activity
Aminooxyacetic acid hemihydrochloride (AOAA) dose-dependently decreases the survival of C6 glioma cells. Aminooxyacetic acid hemihydrochloride treatment produces a significant increase in the percentage of the cells arrested in the stage of G0/G1, as well as a significant decrease in the percentage of the cells at S phase and G2/M phase. Aminooxyacetic acid hemihydrochloride treatment leads to an obvious decrease in the number of the cells in the phase of cell division. Aminooxyacetic acid hemihydrochloride significantly increases the percentage of the cells in both early-stage apoptosis and necrosis. Treatment of the cells with 1 mM or 5 mM Aminooxyacetic acid hemihydrochloride leads to decreased levels of aging of the cells[1]. Glutamine-dependent cell lines show greater inhibition of cell growth by Aminooxyacetic acid hemihydrochloride (AOA) compare with cells that are less glutamine dependent[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2921-14-4
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Appearance Solid
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Molecular Weight 109.30
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Formula C2H5NO3.1/2HCl
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Color White to off-white
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SMILES
OC(CON)=O.[H]Cl.[1/2]
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Synonyms
Carboxymethoxylamine hemihydrochloride; Aminooxyacetate hemihydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (25)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
2023 Feb 15;8(1):65. PMID: 36788227
Aminooxyacetic acid hemihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2023 Feb 15;8(1):65. [Abstract]
Aminooxyacetic acid hemihydrochloride (AOA) (20 μM; 48 h) decreased the migration and invasion of HCC cells expressing p5372P variant.
Aminooxyacetic acid hemihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2023 Feb 15;8(1):65. [Abstract]
A Parental or PSAT1 knockout PLC/PRF/5 cells were tail intravenously injected into BALB/c nude mice and the mice were treated with vehicle or Aminooxyacetic acid hemihydrochloride (AOA) (10 mg/kg; i.p.; once daily for 14 d). Aminooxyacetic acid hemihydrochloride (AOA) treatment significantly inhibited the lung metastasis of cancer cells expressing the p5372P variant in vivo.
Aminooxyacetic acid hemihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2023 Feb 15;8(1):65. [Abstract]
The interaction between PSAT1 and p53 was diminished by Aminooxyacetic acid hemihydrochloride (AOA) (10-20 μM) treatment while the protein level of PSAT1 was not significantly changed.
Aminooxyacetic acid hemihydrochloride purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2023 Feb 15;8(1):65. [Abstract]
Cell viability of Hep3B cells transfected with vector, p53-72P, and p53-72R plasmids was assessed. Cells were treated with regorafenib alone or Aminooxyacetic acid hemihydrochloride (AOA) (10 μM) plus regorafenib at indicated concentrations. Aminooxyacetic acid hemihydrochloride (AOA) sensitized tumor cells containing vector and p53-72P to regorafenib, while exhibiting no obvious synergistic function with regorafenib in cells containing p53-72R.
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Nat Metab
2022 Feb;4(2):239-253. PMID: 35145325
Aminooxyacetic acid hemihydrochloride purchased from MedChemExpress. Usage Cited in: Nat Metab. 2022 Feb;4(2):239-253. [Abstract]
Aminooxyacetic acid hemihydrochloride (GOT1) (1 mM; 2 d). Cell proliferation of H460 cells was quantified after treatment with inhibitors against the indicated enzymes.
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Cancer Res
GOT2 Silencing Promotes Reprogramming of Glutamine Metabolism and Sensitizes Hepatocellular Carcinoma to Glutaminase Inhibitors. [Abstract]2022 Sep 16;82(18):3223-3235. PMID: 35895805 -
Redox Biol
Enteric coronavirus PDCoV evokes a non-Warburg effect by hijacking pyruvic acid as a metabolic hub. [Abstract]2024 May:71:103112. PMID: 38461791 -
Theranostics
Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. [Abstract]2021 Mar 24;11(12):5650-5674. PMID: 33897873 -
Sci Adv
Glutamate utilization fuels rapid production of mitochondrial ROS in dendritic cells and drives systemic inflammation during tularemia. [Abstract]2025 Aug 29;11(35):eadu6271. PMID: 40880474 -
Sci Adv
The small-molecule pifithrin-α deactivates ETR1 to repress shade avoidance in Arabidopsis. [Abstract]2025 Aug 15;11(33):eadw9241. PMID: 40802772 -
Research (Wash D C)
Targeting the Exonic Circular OGT RNA/O-GlcNAc Transferase/Forkhead Box C1 Axis Inhibits Asparagine- and Alanine-Mediated Ferroptosis Repression in Neuroblastoma Progression. [Abstract]2025 May 23:8:0703. PMID: 40416363 -
Phytomedicine
Salvianolic acid A targets glutamic-oxaloacetic transaminase 2 to ameliorate doxorubicin-induced myocardial oxidative injury by activating malate-aspartate NADH shuttle. [Abstract]2025 Nov 25:148:157492. PMID: 41192254 -
Cell Death Discov
Metabolic interplay between exogenous cystine and glutamine dependence in triple-negative breast cancer. [Abstract]2025 Oct 6;11(1):430. PMID: 41053011 -
Phytother Res
Targeting FOXM1/Cystathionine-β-Synthase Axis by Brusatol Inhibits Lung Cancer Malignant Progression. [Abstract]2026 May;40(5):2569-2587. PMID: 41719094 -
ACS Appl Mater Interfaces
Mitochondrial-Targeting Drug-Loaded Nanoparticles Reprogram Macrophage Metabolism via ROS/NO Co-elimination for Diabetic Wound Healing. [Abstract]2025 Dec 24;17(51):69180-69195. PMID: 41383171 -
Stem Cell Res Ther
The CBS/H2S axis regulates intestinal stem cell homeostasis and radiation-induced intestinal damage. [Abstract]2025 Oct 31;16(1):603. PMID: 41174798 -
CNS Neurosci Ther
Alpha-Asarone modulates kynurenine disposal in muscle and mediates resilience to stress-induced depression via PGC-1α induction. [Abstract]2023 Mar;29(3):941-956. PMID: 36575869 -
Cell Mol Life Sci
2024 May 3;81(1):204. PMID: 38700532 -
Mol Nutr Food Res
Agaricus blazei Murrill Polysaccharide Attenuates Periodontitis via H2 S/NRF2 Axis-Boosted Appropriate Level of Autophagy in PDLCs. [Abstract]2023 Nov;67(22):e2300112. PMID: 37775336 -
ASN Neuro
Astrocytic Hydrogen Sulfide Regulates Supraoptic Cellular Activity in the Adaptive Response of Lactating Rats to Chronic Social Stress. [Abstract]2021 Jan-Dec:13:17590914211043087. PMID: 34579557 -
FASEB J
Maternal LDHB Safeguards Redox Balance and Developmental Competence During Preimplantation Embryo Cleavage. [Abstract]2026 May 31;40(10):e71888. PMID: 42117986 -
J Virol
African swine fever virus hijacks host pyrimidine metabolism to promote viral replication. [Abstract]2025 Nov 25;99(11):e0098525. PMID: 41171159 -
Viruses
Asparagine Availability Is a Critical Limiting Factor for Infectious Spleen and Kidney Necrosis Virus Replication. [Abstract]2024 Sep 29;16(10):1540. PMID: 39459874 -
Am J Transl Res
H2S promotes proliferation of endometrial stromal cells via activating the NF-κB pathway in endometriosis. [Abstract]2018 Dec 15;10(12):4247-4257. PMID: 30662667 -
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Biomed Pharmacother
Aminooxyacetic acid hemihydrochloride leads to decreased intracellular ATP levels and altered cell cycle of prostate cancer cells by suppressing energy metabolism. [Abstract]2023 Nov:167:115605. PMID: 37801901 -
Comput Biol Med
System analysis based on glutamine catabolic-related enzymes identifies GPT2 as a novel immunotherapy target for lung adenocarcinoma. [Abstract]2023 Oct:165:107415. PMID: 37657356
Solvent & Solubility
DMSO : 125 mg/mL (1143.64 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (914.91 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (19.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (19.03 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 130 mg/mL (1189.39 mM); Clear solution; Need ultrasonic
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
Enzyme activity of aspartate transaminase is measured by a colorimetric assay assessing formation of pyruvate from oxaloacetate, a product of GOT1/2 (also called AST1/2) activity, as described previously. In brief, cells grown in 6-well plates are collected after 6, 24, or 48 hours of Aminooxyacetic acid hemihydrochloride (AOA) treatment and washed with cold PBS, lysed, and supernatant used for analysis[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Breast cancer cell lines are used in this study. Cells are plated in 96-well plates at 1,500 to 5,000 cells per well in 100 μL media. New medium with varying concentration of Aminooxyacetic acid hemihydrochloride (AOA) is added after 12 hours. The assay is performed after 48 hours[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Female albino rats (150 to 200 g) bred at our department are used. Aminooxyacetic acid hemihydrochloride (AOAA) is injected into a tail vein (2 mL/kg body weight). For this purpose the rat is placed in a plastic tube and the tail warmed in water 42°C[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (278 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Wang C, et al. Malate-aspartate shuttle inhibitor aminooxyacetic acid leads to decreased intracellular ATP levels and altered cell cycle of C6 glioma cells by inhibiting glycolysis. Cancer Lett. 2016 Aug 1;378(1):1-7. [Content Brief]
[2]. Pagliusi SR, et al. Aminooxyacetic acid induced accumulation of GABA in the rat brain. Interaction with GABA receptors and distribution in compartments. Naunyn Schmiedebergs Arch Pharmacol. 1983 Apr;322(3):210-5. [Content Brief]
[3]. Korangath P, et al. Targeting Glutamine Metabolism in Breast Cancer with Aminooxyacetate. Clin Cancer Res. 2015 Jul 15;21(14):3263-73. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 9.1491 mL | 45.7457 mL | 91.4913 mL | 228.7283 mL |
| 5 mM | 1.8298 mL | 9.1491 mL | 18.2983 mL | 45.7457 mL | |
| 10 mM | 0.9149 mL | 4.5746 mL | 9.1491 mL | 22.8728 mL | |
| 15 mM | 0.6099 mL | 3.0497 mL | 6.0994 mL | 15.2486 mL | |
| 20 mM | 0.4575 mL | 2.2873 mL | 4.5746 mL | 11.4364 mL | |
| 25 mM | 0.3660 mL | 1.8298 mL | 3.6597 mL | 9.1491 mL | |
| 30 mM | 0.3050 mL | 1.5249 mL | 3.0497 mL | 7.6243 mL | |
| 40 mM | 0.2287 mL | 1.1436 mL | 2.2873 mL | 5.7182 mL | |
| 50 mM | 0.1830 mL | 0.9149 mL | 1.8298 mL | 4.5746 mL | |
| 60 mM | 0.1525 mL | 0.7624 mL | 1.5249 mL | 3.8121 mL | |
| 80 mM | 0.1144 mL | 0.5718 mL | 1.1436 mL | 2.8591 mL | |
| 100 mM | 0.0915 mL | 0.4575 mL | 0.9149 mL | 2.2873 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Aminooxyacetic acid hemihydrochloride Related Classifications
HY-107994 Related Classifications
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