4-Hydrazino-7-nitro-benzofurazan hydrazine adduct
Based on 1 Customer Validation
4-Hydrazino-7-nitro-benzofurazan hydrazine adduct (NBD-H) is a fluorescent reagent used for protein labeling.
For research use only. We do not sell to patients.
- Purity : 99.83%
- CAS No.: 131467-87-3
- Formula: C6H9N7O3
- Molecular Weight:227.18
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Phthalaldehyde
Biological Activity
Description
In Vitro
Chemical Information
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CAS No. 131467-87-3
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Appearance Solid
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Molecular Weight 227.18
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Formula C6H9N7O3
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Color Brown to dark brown
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SMILES
O=[N+](C1=CC=C(NN)C2=NON=C21)[O-].NN
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Synonyms
NBD-H
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (220.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 10 mg/mL (44.02 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 4.4018 mL | 22.0090 mL | 44.0180 mL | 110.0449 mL |
| 5 mM | 0.8804 mL | 4.4018 mL | 8.8036 mL | 22.0090 mL | |
| 10 mM | 0.4402 mL | 2.2009 mL | 4.4018 mL | 11.0045 mL | |
| 15 mM | 0.2935 mL | 1.4673 mL | 2.9345 mL | 7.3363 mL | |
| 20 mM | 0.2201 mL | 1.1004 mL | 2.2009 mL | 5.5022 mL | |
| 25 mM | 0.1761 mL | 0.8804 mL | 1.7607 mL | 4.4018 mL | |
| 30 mM | 0.1467 mL | 0.7336 mL | 1.4673 mL | 3.6682 mL | |
| 40 mM | 0.1100 mL | 0.5502 mL | 1.1004 mL | 2.7511 mL | |
| DMSO | 50 mM | 0.0880 mL | 0.4402 mL | 0.8804 mL | 2.2009 mL |
| 60 mM | 0.0734 mL | 0.3668 mL | 0.7336 mL | 1.8341 mL | |
| 80 mM | 0.0550 mL | 0.2751 mL | 0.5502 mL | 1.3756 mL | |
| 100 mM | 0.0440 mL | 0.2201 mL | 0.4402 mL | 1.1004 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
4-Hydrazino-7-nitro-benzofurazan hydrazine adduct Related Classifications
HY-W273413 Related Classifications
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