p53 and MDM2 proteins-interaction-inhibitor (chiral)
Based on 1 Customer Validation
p53 and MDM2 protein-interaction inhibitor (chiral) (Compound 32) is an inhibitor of the interaction between p53 and MDM2. p53 and MDM2 protein-interaction inhibitor (chiral) has potential applications in cancer research.
For research use only. We do not sell to patients.
- Purity: 98.13%
- CAS No.: 939981-37-0
- Formula: C40H49Cl2N5O4
- Molecular Weight:734.75
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
0.6 μM
Compound: 2k
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Cytotoxicity against human HCT116 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
Cytotoxicity against human HCT116 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
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[PMID: 24900694] |
| MDA-MB-435 | IC50 |
17.5 μM
Compound: 2k
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Cytotoxicity against human MDA-MB-435 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay
Cytotoxicity against human MDA-MB-435 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay
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[PMID: 24900694] |
| RKO | IC50 |
0.6 μM
Compound: 2k
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Cytotoxicity against human RKO cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
Cytotoxicity against human RKO cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
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[PMID: 24900694] |
| SJSA-1 | IC50 |
0.2 μM
Compound: 2k
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Cytotoxicity against human SJSA1 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
Cytotoxicity against human SJSA1 cells expressing wild type p53 assessed as cell viability after 5 days by MTT assay
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[PMID: 24900694] |
| SW480 | IC50 |
14.4 μM
Compound: 2k
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Cytotoxicity against human SW480 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay
Cytotoxicity against human SW480 cells expressing p53 mutant assessed as cell viability after 5 days by MTT assay
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[PMID: 24900694] |
The anticancer activity of p53 protein is often impaired by the overexpression of oncoprotein Mdm2 and its homolog MdmX. Therefore, disrupting the abnormal p53-MdmX/Mdm2 interaction can restore the activity of p53.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 939981-37-0
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Appearance Solid
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Molecular Weight 734.75
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Formula C40H49Cl2N5O4
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Color White to off-white
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SMILES
O=C(N1CCN(CC(N2CCOCC2)=O)CC1)N3[C@](C)(C4=CC=C(Cl)C=C4)[C@](C)(C5=CC=C(Cl)C=C5)N=C3C6=CC=C(C(C)(C)C)C=C6OCC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 50 mg/mL (68.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.25 mg/mL (4.42 mM); Clear solution
This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3610 mL | 6.8050 mL | 13.6101 mL | 34.0252 mL |
| 5 mM | 0.2722 mL | 1.3610 mL | 2.7220 mL | 6.8050 mL | |
| 10 mM | 0.1361 mL | 0.6805 mL | 1.3610 mL | 3.4025 mL | |
| 15 mM | 0.0907 mL | 0.4537 mL | 0.9073 mL | 2.2683 mL | |
| 20 mM | 0.0681 mL | 0.3403 mL | 0.6805 mL | 1.7013 mL | |
| 25 mM | 0.0544 mL | 0.2722 mL | 0.5444 mL | 1.3610 mL | |
| 30 mM | 0.0454 mL | 0.2268 mL | 0.4537 mL | 1.1342 mL | |
| 40 mM | 0.0340 mL | 0.1701 mL | 0.3403 mL | 0.8506 mL | |
| 50 mM | 0.0272 mL | 0.1361 mL | 0.2722 mL | 0.6805 mL | |
| 60 mM | 0.0227 mL | 0.1134 mL | 0.2268 mL | 0.5671 mL |
p53 and MDM2 proteins-interaction-inhibitor (chiral) Related Classifications
HY-70027 Related Classifications
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