p53 and MDM2 proteins-interaction-inhibitor dihydrochloride
Based on 1 Customer Validation
p53 and MDM2 proteins-interaction-inhibitor dihydrochloride (Compound 32) is an inhibitor of the interaction between p53 and MDM2. p53 and MDM2 proteins-interaction-inhibitor dihydrochloride has potential applications in cancer research.
For research use only. We do not sell to patients.
- Purity : 99.45%
- CAS No.: 2070009-27-5
- Formula: C40H51Cl4N5O4
- Molecular Weight:807.68
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
The anticancer activity of p53 protein is often impaired by the overexpression of oncoprotein Mdm2 and its homolog MdmX. Therefore, disrupting the abnormal p53-MdmX/Mdm2 interaction can restore the activity of p53.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2070009-27-5
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Appearance Solid
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Molecular Weight 807.68
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Formula C40H51Cl4N5O4
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Color White to off-white
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SMILES
O=C(N1CCN(CC(N2CCOCC2)=O)CC1)N3[C@](C)(C4=CC=C(Cl)C=C4)[C@](C)(C5=CC=C(Cl)C=C5)N=C3C6=CC=C(C(C)(C)C)C=C6OCC.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (123.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.10 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.10 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.2381 mL | 6.1906 mL | 12.3811 mL | 30.9529 mL |
| 5 mM | 0.2476 mL | 1.2381 mL | 2.4762 mL | 6.1906 mL | |
| 10 mM | 0.1238 mL | 0.6191 mL | 1.2381 mL | 3.0953 mL | |
| 15 mM | 0.0825 mL | 0.4127 mL | 0.8254 mL | 2.0635 mL | |
| 20 mM | 0.0619 mL | 0.3095 mL | 0.6191 mL | 1.5476 mL | |
| 25 mM | 0.0495 mL | 0.2476 mL | 0.4952 mL | 1.2381 mL | |
| 30 mM | 0.0413 mL | 0.2064 mL | 0.4127 mL | 1.0318 mL | |
| 40 mM | 0.0310 mL | 0.1548 mL | 0.3095 mL | 0.7738 mL | |
| 50 mM | 0.0248 mL | 0.1238 mL | 0.2476 mL | 0.6191 mL | |
| 60 mM | 0.0206 mL | 0.1032 mL | 0.2064 mL | 0.5159 mL | |
| 80 mM | 0.0155 mL | 0.0774 mL | 0.1548 mL | 0.3869 mL | |
| 100 mM | 0.0124 mL | 0.0619 mL | 0.1238 mL | 0.3095 mL |
p53 and MDM2 proteins-interaction-inhibitor dihydrochloride Related Classifications
HY-70027A Related Classifications
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