Pararosaniline hydrochloride
Based on 1 Customer Validation
Pararosaniline hydrochloride (Basic red 9) is a pH-responsive basic dye, as a biological stain to track certain proteins. The pH of the acidified Pararosaniline hydrochloride reagent has a significant effect on the color and the maximum absorption wavelength (λmax) of the reaction system, with its optimum pH 0.48 and a λmax at 549 nm. Pararosaniline hydrochloride is also a strong modifier of RNA splicing. Pararosaniline hydrochloride has been used in the analysis of SO2 and formaldehyde and staining of bacteria or other organisms. Pararosaniline hydrochloride is extensively used in industries like textile, printing, paper, cosmetic, and leather.
For research use only. We do not sell to patients.
- Purity: 97.33%
- CAS No.: 569-61-9
- Formula: C19H18ClN3
- Molecular Weight:323.83
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Storage:
RT, sealed storage, away from moisture and light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Pararosaniline hydrochloride (1 mM, 24 h) is deleterious to C. elegans physiology and causes the upregulation of the rbm-17 gene[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 569-61-9
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Appearance Solid
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Molecular Weight 323.83
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Formula C19H18ClN3
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Color Green to dark green
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SMILES
N=C(C=C/1)C=CC1=C(C2=CC=C(N)C=C2)\C3=CC=C(N)C=C3.Cl
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Synonyms
Basic red 9
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
RT, sealed storage, away from moisture and light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 50 mg/mL (154.40 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 9.46 mg/mL (29.21 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (275 KB)
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SDS (418 KB)
- English - EN (418 KB)
- Français - FR (418 KB)
- Deutsch - DE (418 KB)
- Norwegian - NO (418 KB)
- Español - ES (418 KB)
- Swedish - SV (418 KB)
- Italian - IT (418 KB)
- Korean - KR (418 KB)
- Portuguese - PT (418 KB)
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Handling Instructions (2659 KB)
References
[1]. Yaagoob IY, et al. A resin containing motifs of maleic acid and glycine: a super-adsorbent for adsorptive removal of basic dye pararosaniline hydrochloride and Cd(II) from water. J Environ Health Sci Eng. 2021 Jun 17;19(2):1333-1346. [Content Brief]
[3]. Huynh D, et al. Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans. G3 (Bethesda). 2023 Dec 6;13(12):jkad241. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.0880 mL | 15.4402 mL | 30.8804 mL | 77.2010 mL |
| 5 mM | 0.6176 mL | 3.0880 mL | 6.1761 mL | 15.4402 mL | |
| 10 mM | 0.3088 mL | 1.5440 mL | 3.0880 mL | 7.7201 mL | |
| 15 mM | 0.2059 mL | 1.0293 mL | 2.0587 mL | 5.1467 mL | |
| 20 mM | 0.1544 mL | 0.7720 mL | 1.5440 mL | 3.8601 mL | |
| 25 mM | 0.1235 mL | 0.6176 mL | 1.2352 mL | 3.0880 mL | |
| DMSO | 30 mM | 0.1029 mL | 0.5147 mL | 1.0293 mL | 2.5734 mL |
| 40 mM | 0.0772 mL | 0.3860 mL | 0.7720 mL | 1.9300 mL | |
| 50 mM | 0.0618 mL | 0.3088 mL | 0.6176 mL | 1.5440 mL | |
| 60 mM | 0.0515 mL | 0.2573 mL | 0.5147 mL | 1.2867 mL | |
| 80 mM | 0.0386 mL | 0.1930 mL | 0.3860 mL | 0.9650 mL | |
| 100 mM | 0.0309 mL | 0.1544 mL | 0.3088 mL | 0.7720 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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