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  3. Phenacetin

Phenacetin  (Synonyms: Acetophenetidin)

Cat. No.: HY-B0476 Purity: 99.74%
COA Handling Instructions

Phenacetin (Acetophenetidin) is a non-opioid analgesic/antipyretic agent. Phenacetin is a selective COX-3 inhibitor. Phenacetin is used as probe of cytochrome P450 enzymes CYP1A2 in human liver microsomes and in rats.

For research use only. We do not sell to patients.

Phenacetin Chemical Structure

Phenacetin Chemical Structure

CAS No. : 62-44-2

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10 mM * 1 mL in DMSO USD 61 In-stock
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Description

Phenacetin (Acetophenetidin) is a non-opioid analgesic/antipyretic agent. Phenacetin is a selective COX-3 inhibitor. Phenacetin is used as probe of cytochrome P450 enzymes CYP1A2 in human liver microsomes and in rats[1][2][3].

IC50 & Target

COX-3

102 μM (IC50)

In Vitro

Phenacetin shows inhibitory activity for COX-3 with an IC50 value of 102 μM in insect cells [1].
Phenacetin is a substrate widely used as an in vitro and in vivo probe to measure the activity of CYP1A2, because the metabolism of phenacetin to acetaminophen is thought to be a selective CYP1A2-mediated reaction[2][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Phenacetin exhibits elimination half-life (rat 2.06 h) and Cmax (6,728.3 μg/L) following oral administration (rat 20 mg/kg)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (180-200 g)[2]
Dosage: 20 mg/kg
Administration: Oral administration (Pharmacokinetic Analysis)
Result: T1/2 (2.06 hours), Cmax (6,728.3 μg/L).
Molecular Weight

179.22

Formula

C10H13NO2

CAS No.
SMILES

CC(NC1=CC=C(OCC)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (557.97 mM)

H2O : 0.67 mg/mL (3.74 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.5797 mL 27.8987 mL 55.7973 mL
5 mM 1.1159 mL 5.5797 mL 11.1595 mL
10 mM 0.5580 mL 2.7899 mL 5.5797 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (13.95 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.74%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Phenacetin
Cat. No.:
HY-B0476
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