Phenothiazine
Based on 4 publication(s) in Google Scholar
Phenothiazine is an antibiotic which has insecticidal, fungicidal, antibacterial and anthelmintic activities. Phenothiazine also can be used for the research of neurological diseases.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 92-84-2
- Formula: C12H9NS
- Molecular Weight:199.28
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Anti-Infection Compound Library
HY-L002
-
FDA-Approved Drug Library
HY-L022
-
Anti-Aging Compound Library
HY-L034
-
Drug Repurposing Compound Library
HY-L035
-
Oxygen Sensing Compound Library
HY-L045
-
Antifungal Compound Library
HY-L048
-
Antibacterial Compound Library
HY-L049
-
Ferroptosis Compound Library
HY-L051
-
FDA Approved & Pharmacopeial Drug Library
HY-L066
-
Antibiotics Library
HY-L067
-
Neuroprotective Compound Library
HY-L070
-
Antiparasitic Compound library
HY-L082
-
Food-Sourced Compound Library
HY-L094
-
Rare Diseases Drug Library
HY-L102
-
EMA-Approved Drug Library
HY-L116
-
Human Metabolite Library
HY-L123
-
Off-patent Drug Library
HY-L141
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Drug Repurposing Compound Library Plus
HY-L035P
-
FDA-Approved Drug Library Plus
HY-L022P
-
FDA-Approved Drug Library Mini
HY-L022M
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
-
Mass Spectrometry Human Metabolite Library
HY-L215
-
Environmental Pollutant Compound Library
HY-L247
-
Drug Fragment Library
HY-L904
Publications Citing Use of MedChemExpress (MCE) Phenothiazine
MoreAll Antibiotic Isoforms
MoreAll Parasite Isoforms
More
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| HT-1080 | EC50 |
0.049 μM
Compound: phenothiazine
|
Inhibition of erastin-induced ferroptosis in human HT-1080 cells assessed as cell viability incubated for 48 hrs by MTT assay
Inhibition of erastin-induced ferroptosis in human HT-1080 cells assessed as cell viability incubated for 48 hrs by MTT assay
|
[PMID: 33065375] |
| K562 | IC50 |
>100 μM
Compound: 9
|
Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis
Antiproliferative activity against human K562 cells after 48 hrs by hemocytometric analysis
|
[PMID: 21563750] |
| RBL-1 | IC50 |
0.94 μM
Compound: 29
|
In vitro inhibition of Prostaglandin G/H synthase pathway in rat basophilic leukemia (RBL-1) cells
In vitro inhibition of Prostaglandin G/H synthase pathway in rat basophilic leukemia (RBL-1) cells
|
[PMID: 2113951] |
| RBL-1 | IC50 |
1.4 μM
Compound: 29
|
In vitro inhibition of 5-lipoxygenase pathway in rat basophilic leukemia (RBL-1) cells
In vitro inhibition of 5-lipoxygenase pathway in rat basophilic leukemia (RBL-1) cells
|
[PMID: 2113951] |
| U-937 | IC50 |
1.39 μM
Compound: 9
|
Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay
Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay
|
[PMID: 24900465] |
Phenothiazine inhibits cell death caused by oxidative stress in cells HT22 (causes by glutamate, EC50=0.02 μM), SK-N-MC (causes by H2O2, EC50=0.02 μM)and SH-SY5Y (causes by MPP+, EC50=0.02 μM). Phenothiazine inhibits lipid peroxidation in Ascorbate (HY-B0166)-damaged pig brain membranes, exhibits antioxidant efficacy[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Phenothiazine protects dopaminergic neurons from the toxic effects of MPP+ and Rotenone (HY-B1756) in C. elegans models[3].
Phenothiazine (5-30 mg/kg, sc, single dose) reduces brain lesions, and exhibits neuroprotective efficacy in traumatic brain injury (TBI) mouse models[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Traumatic brain injury (TBI) mouse models[4]
-
Dosage:5-30 mg/kg
-
Administration:sc, single dose
-
Result:Reduced brain lesions and weight loss.
Chemical Information
-
CAS No. 92-84-2
-
Appearance Solid
-
Molecular Weight 199.28
-
Formula C12H9NS
-
Color Off-white to gray
-
SMILES
C12=CC=CC=C1NC3=C(C=CC=C3)S2
-
Synonyms
Thiodiphenylamine
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
-
Journal Impact Factor
-
Most Recent
-
Cell Commun Signal
Tricyclic antidepressants induce liver inflammation by targeting NLRP3 inflammasome activation. [Abstract]2023 May 25;21(1):123. PMID: 37231437 -
ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Cell Tissue Res
Phenothiazine alleviates cisplatin-mediated acute kidney injury by inhibiting ferroptosis. [Abstract]2026 Jan 23;403(1):12. PMID: 41575591
Solvent & Solubility
DMSO : 100 mg/mL (501.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.55 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (277 KB)
-
SDS (761 KB)
- English - EN (761 KB)
- Français - FR (761 KB)
- Deutsch - DE (761 KB)
- Norwegian - NO (761 KB)
- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
-
Handling Instructions (2659 KB)
References
[1]. Jun-hua Xiao, et al. Xiao JH, Zhang YL, Ding LL, Feng XL, Wang JL. Effects of isoliensinine on proliferation of porcine coronary arterial smooth muscle cells induced by phenylephrine. Yao Xue Xue Bao. 2005 Feb;40(2):105-10. [Content Brief]
[2]. Mitchell SC, et, al. The toxicity of phenothiazine. Drug Metabol Drug Interact. 1994;11(3):201-35. [Content Brief]
[4]. Songarj P, et al., The antioxidative, non-psychoactive tricyclic phenothiazine reduces brain damage after experimental traumatic brain injury in mice. Neurosci Lett. 2015 Jan 1;584:253-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.0181 mL | 25.0903 mL | 50.1807 mL | 125.4516 mL |
| 5 mM | 1.0036 mL | 5.0181 mL | 10.0361 mL | 25.0903 mL | |
| 10 mM | 0.5018 mL | 2.5090 mL | 5.0181 mL | 12.5452 mL | |
| 15 mM | 0.3345 mL | 1.6727 mL | 3.3454 mL | 8.3634 mL | |
| 20 mM | 0.2509 mL | 1.2545 mL | 2.5090 mL | 6.2726 mL | |
| 25 mM | 0.2007 mL | 1.0036 mL | 2.0072 mL | 5.0181 mL | |
| 30 mM | 0.1673 mL | 0.8363 mL | 1.6727 mL | 4.1817 mL | |
| 40 mM | 0.1255 mL | 0.6273 mL | 1.2545 mL | 3.1363 mL | |
| 50 mM | 0.1004 mL | 0.5018 mL | 1.0036 mL | 2.5090 mL | |
| 60 mM | 0.0836 mL | 0.4182 mL | 0.8363 mL | 2.0909 mL | |
| 80 mM | 0.0627 mL | 0.3136 mL | 0.6273 mL | 1.5681 mL | |
| 100 mM | 0.0502 mL | 0.2509 mL | 0.5018 mL | 1.2545 mL |
Powered by Bioz