Amelioration of myocarditis by HVEM-overexpressing dendritic cells through induction of IL-10-producing cells

  • Cardiovasc Res. 2009 Dec 1;84(3):425-33. doi: 10.1093/cvr/cvp219.
Gang Cai  1 ,  Huaizhou Wang ,  Qin Qin ,  Jun Zhang ,  Zhi Zhu ,  Menglei Liu ,  Qian Shen
Affiliations
  • 1. Department of Experimental Diagnosis, Shanghai Changhai Hospital, Shanghai 200433, People's Republic of China.
PMID: 19570810 DOI: 10.1093/cvr/cvp219
Abstract

Aims: Herpes virus entry mediator (HVEM) is considered to be a molecular 'switch' for immune responses, and a role in immune modification has been reported. The aim of this study was to assess whether HVEM-mediated immune suppression could protect against experimental autoimmune myocarditis (EAM) induced by Myosin.

Methods and results: We constructed HVEM-expressing adenovirus (AdHVEM) and fusion protein HVEM-Ig and evaluated their roles in immunoregulation in vitro and in vivo. Immunoregulation of dendritic cells (DCs) infected with Recombinant virus or treated with HVEM-Ig was then studied. DCs transfected with AdHVEM (DC-AdHVEM) were protected against EAM, whereas HVEM-Ig had no protective effect. Further study showed that DC-AdHVEMs produced a regulatory cytokine, IL-10, which had further effects on induction of IL-10 producing CD4(+) T cells. This subset of T cells was then responsible for the protection against EAM.

Conclusion: Myosin-DC-AdHVEM cell gene therapy appears to be a safe and effective way of inhibiting the development of EAM. The signal induced by HVEM seems to play different roles in different cells.

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