SERPINA1 promotes the invasion, metastasis, and proliferation of pancreatic ductal adenocarcinoma via the PI3K/Akt/NF-κB pathway
- Biochem Pharmacol. 2024 Dec;230(Pt 2):116580. doi: 10.1016/j.bcp.2024.116580.
- 1. Department of Gastroenterology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
- 2. Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.
- 3. Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. Electronic address: [email protected].
- 4. Department of Gastroenterology, The Fifth Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. Electronic address: [email protected].
Serpin peptidase inhibitor clade A member 1 (SERPINA1) is highly expressed in a variety of solid tumors. However, its role in pancreatic ductal adenocarcinoma (PDAC) remains unclear. Here, we report evidence that SERPINA1 acts as a potent oncogene to drive its extremely malignant character. We found that elevated SERPINA1 expression in primary tumors was associated with lymph node metastasis and shorter survival in PDAC patients. Mechanistic investigations revealed that overexpression of SERPINA1 induced nuclear translocation and phosphorylation of the p65 subunit through the PI3K/Akt/NF-κB pathway, thereby promoting the invasion, metastasis and proliferation of PDAC cells in vitro and in vivo. Conversely, the knockdown of SERPINA1 attenuated this signaling pathway and restored the phenotype of PDAC cells overexpressing SERPINA1. Overall, our study reveals that SERPINA1 affects the properties of PDAC through the PI3K/Akt/NF-κB pathway, and its activation confers the clinical features of epithelial-mesenchymal transition and proliferation in the disease.