ALDH1A3 Protein, Human (His)

ALDH1A3 Protein, Human (His) is a human recombinant ALDH1A3 with a His tag at the N-terminus. ALDH1A3 Protein, Human (His) is produced in E. coli and the target gene encoding Met1-Pro512 is expressed.

Aldehyde Dehydrogenase 1-A3 (ALDH1A3) catalyzes the oxidation of retinal to the pleiotropic factor retinoic acid using NAD+. ALDH1A3 participates in various physiological processes in human cells by oxidizing all-trans-retinal to retinoic acid. ALDH1A3 influences a diverse range of biological characteristics within cancer stem cells and can act as a marker for these cells. ALDH1A3 is differentially activated during early embryonic head and forebrain development. ALDH1A3 is considered a target in breast cancer. High expression of ALDH1A3 has been observed in high-grade gliomas (HGGs)^[1][2].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P47895 (M1-P512)

220  [NCBI]

Full Length

MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP

Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 20% Glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Moretti A, et al. Crystal structure of human aldehyde dehydrogenase 1A3 complexed with NAD+ and retinoic acid. Sci Rep. 2016 Oct 19;6:35710.  [Content Brief]

  [2]. Duan JJ, et al. ALDH1A3, a metabolic target for cancer diagnosis and therapy. Int J Cancer. 2016 Sep 1;139(5):965-75.  [Content Brief]