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Alpha-2-macroglobulin Protein, Human (sf9, His)

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Alpha-2-macroglobulin Protein, a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. A2M acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines. It inhibits inflammatory mediators of chondrocytes by blocking IL-1尾/NF-魏B pathway. Alpha-2-macroglobulin Protein, Human (sf9, His) is the recombinant human-derived Alpha-2-macroglobulin protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Alpha-2-macroglobulin Protein, a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. A2M acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines. It inhibits inflammatory mediators of chondrocytes by blocking IL-1尾/NF-魏B pathway. Alpha-2-macroglobulin Protein, Human (sf9, His) is the recombinant human-derived Alpha-2-macroglobulin protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

Alpha-2-macroglobulin (A2M), a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. It is also a large plasma protein found in the blood. It is mainly produced by the liver, and also locally synthesized by macrophages, fibroblasts, and adrenocortical cells. The inhibitor acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines, including transforming growth factor-beta 1 (TGF-β1), TGF-β2, nerve growth factor-beta, platelet-derived growth factor-BB, tumor necrosis factor-alpha (TNF-α), and basic fibroblast growth factor. A2M inhibits inflammatory mediators of chondrocytes by blocking IL-1β/NF-κB pathway. A2M as a novel disease modifying protein in osteoporosis, downregulation of which in bone marrow promotes SSPC dysfunction and imbalances in skeletal homeostasis[1][2][3].

Verified Bioactivity

Measured by its ability to trap trypsin. The trapped trypsin is no longer able to interact with protein substrates or inhibitors, but still able to cleave small peptide substrates or inhibitors.The IC50 value is <5 nM.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    NP_000005.2 (S24-A1474)

  • Gene ID
    2  [NCBI]
  • Molecular Construction
    • N-term
    • Alpha-2-macroglobulin (S24-A1474)
      Accession # NP_000005.2
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    A2M; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein 5; Alpha-2-Macroglobulin; Alpha-2-M; CPAMD5; Alternative Protein A2M; FWP007; A2MD; S863-7

  • AA Sequence

    SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

  • Predicted Molecular Mass

    164 kDa

  • Molecular Weight

    Approximately 160-170 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris,150 mM NaCl, 20% glycerol, pH 7.4
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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