Alpha-2-macroglobulin Protein, Human (sf9, His)

Alpha-2-macroglobulin Protein, a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. A2M acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines. It inhibits inflammatory mediators of chondrocytes by blocking IL-1尾/NF-魏B pathway. Alpha-2-macroglobulin Protein, Human (sf9, His) is the recombinant human-derived Alpha-2-macroglobulin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Alpha-2-macroglobulin Protein, Human (sf9, His) is 1451 a.a., with molecular weight of 160-170 kDa.

Alpha-2-macroglobulin (A2M), a protease inhibitor and cytokine transporter, belongs to the MEROPS proteinase inhibitor family I39, clan IL. It is also a large plasma protein found in the blood. It is mainly produced by the liver, and also locally synthesized by macrophages, fibroblasts, and adrenocortical cells. The inhibitor acts on all classes of endoproteases and could potentially be used to slow or halt PTOA by neutralizing catabolic cascades in affected cartilage. A2M can enter into cells and bind to numerous cytokines, including transforming growth factor-beta 1 (TGF-β1), TGF-β2, nerve growth factor-beta, platelet-derived growth factor-BB, tumor necrosis factor-alpha (TNF-α), and basic fibroblast growth factor. A2M inhibits inflammatory mediators of chondrocytes by blocking IL-1β/NF-κB pathway. A2M as a novel disease modifying protein in osteoporosis, downregulation of which in bone marrow promotes SSPC dysfunction and imbalances in skeletal homeostasis^[1][2][3].

Measured by its ability to trap trypsin. The trapped trypsin is no longer able to interact with protein substrates or inhibitors, but still able to cleave small peptide substrates or inhibitors.The IC₅₀ value is <5 nM.

Human

Sf9 insect cells

C-His

NP_000005.2 (S24-A1474)

2  [NCBI]

SVSGKPQYMVLVPSLLHTETTEKGCVLLSYLNETVTVSASLESVRGNRSLFTDLEAENDVLHCVAFAVPKSSSNEEVMFLTVQVKGPTQEFKKRTTVMVKNEDSLVFVQTDKSIYKPGQTVKFRVVSMDENFHPLNELIPLVYIQDPKGNRIAQWQSFQLEGGLKQFSFPLSSEPFQGSYKVVVQKKSGGRTEHPFTVEEFVLPKFEVQVTVPKIITILEEEMNVSVCGLYTYGKPVPGHVTVSICRKYSDASDCHGEDSQAFCEKFSGQLNSHGCFYQQVKTKVFQLKRKEYEMKLHTEAQIQEEGTVVELTGRQSSEITRTITKLSFVKVDSHFRQGIPFFGQVRLVDGKGVPIPNKVIFIRGNEANYYSNATTDEHGLVQFSINTTNVMGTSLTVRVNYKDRSPCYGYQWVSEEHEEAHHTAYLVFSPSKSFVHLEPMSHELPCGHTQTVQAHYILNGGTLLGLKKLSFYYLIMAKGGIVRTGTHGLLVKQEDMKGHFSISIPVKSDIAPVARLLIYAVLPTGDVIGDSAKYDVENCLANKVDLSFSPSQSLPASHAHLRVTAAPQSVCALRAVDQSVLLMKPDAELSASSVYNLLPEKDLTGFPGPLNDQDDEDCINRHNVYINGITYTPVSSTNEKDMYSFLEDMGLKAFTNSKIRKPKMCPQLQQYEMHGPEGLRVGFYESDVMGRGHARLVHVEEPHTETVRKYFPETWIWDLVVVNSAGVAEVGVTVPDTITEWKAGAFCLSEDAGLGISSTASLRAFQPFFVELTMPYSVIRGEAFTLKATVLNYLPKCIRVSVQLEASPAFLAVPVEKEQAPHCICANGRQTVSWAVTPKSLGNVNFTVSAEALESQELCGTEVPSVPEHGRKDTVIKPLLVEPEGLEKETTFNSLLCPSGGEVSEELSLKLPPNVVEESARASVSVLGDILGSAMQNTQNLLQMPYGCGEQNMVLFAPNIYVLDYLNETQQLTPEIKSKAIGYLNTGYQRQLNYKHYDGSYSTFGERYGRNQGNTWLTAFVLKTFAQARAYIFIDEAHITQALIWLSQRQKDNGCFRSSGSLLNNAIKGGVEDEVTLSAYITIALLEIPLTVTHPVVRNALFCLESAWKTAQEGDHGSHVYTKALLAYAFALAGNQDKRKEVLKSLNEEAVKKDNSVHWERPQKPKAPVGHFYEPQAPSAEVEMTSYVLLAYLTAQPAPTSEDLTSATNIVKWITKQQNAQGGFSSTQDTVVALHALSKYGAATFTRTGKAAQVTIQSSGTFSSKFQVDNNNRLLLQQVSLPELPGEYSMKVTGEGCVYLQTSLKYNILPEKEEFPFALGVQTLPQTCDEPKAHTSFQISLSVSYTGSRSASNMAIVDVKMVSGFIPLKPTVKMLERSNHVSRTEVSSNHVLIYLDKVSNQTLSLFFTVLQDVPVRDLKPAIVKVYDYYETDEFAIAEYNAPCSKDLGNA

160-170 kDa

Greater than 85% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl, 20% glycerol, pH 7.4

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.