1. Recombinant Proteins
  2. Others
  3. ARF3 Protein, Human (His)

ARF3 Protein, Human (His)

Cat. No.: HY-P75583
COA Handling Instructions

ARF3 protein, a GTP-binding allosteric activator of cholera toxin, plays a role in protein trafficking and vesicle dynamics in the Golgi apparatus. Beyond toxin activation, ARF3 interacts with PRKCABP, participating in diverse cellular processes. It also associates with PI4KB and NCS1/FREQ at the Golgi complex, emphasizing its role in crucial molecular interactions for cellular functions. ARF3 Protein, Human (His) is the recombinant human-derived ARF3 protein, expressed by E. coli , with N-His labeled tag. The total length of ARF3 Protein, Human (His) is 181 a.a., with molecular weight of ~23 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
5 μg $75 In-stock
10 μg $125 In-stock
50 μg $355 In-stock
100 μg $600 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

ARF3 protein, a GTP-binding allosteric activator of cholera toxin, plays a role in protein trafficking and vesicle dynamics in the Golgi apparatus. Beyond toxin activation, ARF3 interacts with PRKCABP, participating in diverse cellular processes. It also associates with PI4KB and NCS1/FREQ at the Golgi complex, emphasizing its role in crucial molecular interactions for cellular functions. ARF3 Protein, Human (His) is the recombinant human-derived ARF3 protein, expressed by E. coli , with N-His labeled tag. The total length of ARF3 Protein, Human (His) is 181 a.a., with molecular weight of ~23 kDa.

Background

ARF3, a GTP-binding protein, acts as an allosteric activator of the catalytic subunit of cholera toxin, an ADP-ribosyltransferase. Beyond its role in toxin activation, ARF3 is implicated in protein trafficking, exerting potential influence on vesicle budding and uncoating within the Golgi apparatus. This multifaceted protein interacts with PRKCABP, highlighting its involvement in various cellular processes. Additionally, ARF3 associates with PI4KB and NCS1/FREQ at the Golgi complex, further emphasizing its participation in molecular interactions crucial for cellular functions.

Species

Human

Source

E. coli

Tag

N-His

Accession

P61204 (M1-K181)

Gene ID

377  [NCBI]

Molecular Construction
N-term
His
ARF3 (M1-K181)
Accession # P61204
C-term
Synonyms
ADP-ribosylation factor 3; ARF3
Molecular Weight

Approximately 23 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 300 mM NaCl, 5% trehalose, 5% mannitol and 0.01% Tween80, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ARF3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
ARF3 Protein, Human (His)
Cat. No.:
HY-P75583
Quantity:
MCE Japan Authorized Agent: