1. Recombinant Proteins
  2. Arginase-1 Protein, Human (HEK293, His)

Arginase-1 Protein, Human (HEK293, His)

Cat. No.: HY-P7602
Handling Instructions

Arginase-1 Protein, Human (HEK293, His) expresses in HEK293 cells with a His tag at the N-terminus. Arginase-1 Protein inhibits the growth of the arginine-depleting enzyme arginine deiminase (ADI)-resistant Hep3B tumor cells in mice[1].

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock Quantity
10 μg $200 In-stock
Estimated Time of Arrival: December 31
50 μg $600 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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  • Technical Parameters

  • Properties

  • Documentation

Synonyms
rHuArginase-1, His; ARG1; Arginase-1
Species

Human

Source

HEK 293

Accession

P05089 (M1-K322)

Gene ID

383

Molecular Weight

Approximately 38.0 kDa

AA Sequence

MSAKSRTIGIIGAPFSKGQPRGGVEEGPTVLRKAGLLEKLKEQECDVKDYGDLPFADIPNDSPFQIVKNPRSVGKASEQLAGKVAEVKKNGRISLVLGGDHSLAIGSISGHARVHPDLGVIWVDAHTDINTPLTTTSGNLHGQPVSFLLKELKGKIPDVPGFSWVTPCISAKDIVYIGLRDVDPGEHYILKTLGIKYFSMTEVDRLGIGKVMEETLSYLLGRKKRPIHLSFDVDGLDPSFTPATGTPVVGGLTYREGLYITEEIYKTGLLSGLDIMEVNPSLGKTPEEVTRTVNTAVAITLACFGLAREGNHKPIDYLNPPKHHHHHH

Purity

Greater than 90% as determined by reducing SDS-PAGE

Biological Activity
Measured by the production of urea during the hydrolysis of arginine. The specific activity is 46411 pmol/min/µg.
Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, 20%Glycerol, 1 mM DTT, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Background

Recombinant Human Arginase-1 has IC50s of 0.18 U/mL and 0.07 U/mL for HepG2 and Hep3B cells. Recombinant Human Arginase-1 has a Km value of 1.9 mM for arginine[1].

Documentation
References
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Arginase-1 Protein, Human (HEK293, His)
Cat. No.:
HY-P7602
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