B7-H2/ICOSLG Protein, Mouse (HEK293, His)

B7-H2/ICOSLG, a crucial ligand for T-cell receptor ICOS, mediates immune responses. It serves as a costimulatory signal, promoting T-cell and B-cell proliferation, cytokine secretion, and plasma cell differentiation. Beyond immune activation, it may influence local tissue responses during inflammation and modulate secondary immune responses. During pregnancy, B7-H2/ICOSLG favors an immunoprotective Th2 phenotype in maternal T-cells. It also interacts with CTLA4, contributing to intricate regulatory pathways of immune function. B7-H2/ICOSLG Protein, Mouse (HEK293, His) is the recombinant mouse-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag. The total length of B7-H2/ICOSLG Protein, Mouse (HEK293, His) is 233 a.a., with molecular weight of ~40-52 kDa.

B7-H2/ICOSLG, a ligand for the T-cell-specific surface receptor ICOS, serves as a crucial mediator of immune responses. This protein functions as a costimulatory signal, promoting T-cell proliferation and cytokine secretion while also inducing B-cell proliferation and differentiation into plasma cells. Beyond its role in immune activation, B7-H2/ICOSLG may play a pivotal role in mediating local tissue responses during inflammatory conditions. Furthermore, it contributes to the modulation of the secondary immune response by co-stimulating memory T-cell function. Notably, during pregnancy, B7-H2/ICOSLG may influence the cytokine profile of maternal T-cells, favoring an immunoprotective Th2 phenotype. Additionally, the interaction of B7-H2/ICOSLG with CTLA4 has been observed in vitro, highlighting its involvement in intricate regulatory pathways of immune function.

1.Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.438 μM as determined in SPR assay (Biacore T200).
2.Immobilized Mouse ICOS at 5 μg/mL (100 μL/well) can bind Mouse B7-H2, the ED₅₀ for this effect is 83.21ng/mL.

Mouse

HEK293

C-His

Q9JHJ8-1 (E47-K279)

50723  [NCBI]

ETEVGAMVGSNVVLSCIDPHRRHFNLSGLYVYWQIENPEVSVTYYLPYKSPGINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRVFMNTATELVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLYWINTTDNSLIDTALQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENVALHQNITSISQAESFTGNNTKNPQETHNNELK

Approximately 40-52 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.