B7-H2/ICOSLG Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
B7-H2/ICOSLG is an important ligand of the T cell receptor ICOS and mediates immune responses. It acts as a costimulatory signal, promoting T and B cell proliferation, cytokine secretion, and plasma cell differentiation. B7-H2/ICOSLG Protein, Mouse (HEK293, His) is the recombinant mouse-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
B7-H2/ICOSLG is an important ligand of the T cell receptor ICOS and mediates immune responses. It acts as a costimulatory signal, promoting T and B cell proliferation, cytokine secretion, and plasma cell differentiation. B7-H2/ICOSLG Protein, Mouse (HEK293, His) is the recombinant mouse-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-His labeled tag.
Background
B7-H2/ICOSLG, a ligand for the T-cell-specific surface receptor ICOS, serves as a crucial mediator of immune responses. This protein functions as a costimulatory signal, promoting T-cell proliferation and cytokine secretion while also inducing B-cell proliferation and differentiation into plasma cells. Beyond its role in immune activation, B7-H2/ICOSLG may play a pivotal role in mediating local tissue responses during inflammatory conditions. Furthermore, it contributes to the modulation of the secondary immune response by co-stimulating memory T-cell function. Notably, during pregnancy, B7-H2/ICOSLG may influence the cytokine profile of maternal T-cells, favoring an immunoprotective Th2 phenotype. Additionally, the interaction of B7-H2/ICOSLG with CTLA4 has been observed in vitro, highlighting its involvement in intricate regulatory pathways of immune function.
Verified Bioactivity
1.Mouse ICOS, hFc Tag captured on CM5 Chip via Protein A can bind Mouse B7-H2, His Tag with an affinity constant of 0.438 μM as determined in SPR assay (Biacore T200).
2.Immobilized Mouse ICOS at 5 μg/mL (100 μL/well) can bind Mouse B7-H2, the ED50 for this effect is 83.21ng/mL.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
Q9JHJ8-1 (E47-K279)
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Molecular Construction
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N-term
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B7-H2 (E47-K279)
Accession # Q9JHJ8-1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ICOSLG; KIAA0653; Prev. ICOSL; CD275; B7-Related Protein 1; GL50; B7 Homolog 2; B7h; B7RP-1; B7 Homologue 2; B7RP1; Transmembrane Protein B7-H2 ICOS Ligand; B7-H2; Inducible T-Cell Costimulator Ligand; B7H2; CD275 Antigen; B7-Like Protein Gl50; IMD119; IC
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AA Sequence
ETEVGAMVGSNVVLSCIDPHRRHFNLSGLYVYWQIENPEVSVTYYLPYKSPGINVDSSYKNRGHLSLDSMKQGNFSLYLKNVTPQDTQEFTCRVFMNTATELVKILEEVVRLRVAANFSTPVISTSDSSNPGQERTYTCMSKNGYPEPNLYWINTTDNSLIDTALQNNTVYLNKLGLYDVISTLRLPWTSRGDVLCCVENVALHQNITSISQAESFTGNNTKNPQETHNNELK
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Molecular Weight
Approximately 45-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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