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BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag)

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BAFF/TNFSF13B protein is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway together with TNFSF13/APRIL. These interactions play a crucial role in stimulating B-cell and T-cell function and regulating humoral immunity. BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) is the recombinant human-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Flag labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag)
  • BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

BAFF/TNFSF13B protein is a cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway together with TNFSF13/APRIL. These interactions play a crucial role in stimulating B-cell and T-cell function and regulating humoral immunity. BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) is the recombinant human-derived BAFF/TNFSF13B protein, expressed by HEK293 , with N-His, N-Flag labeled tag.

Background

BAFF/TNFSF13B protein, a cytokine, binds to TNFRSF13B/TACI and TNFRSF17/BCMA, forming a key ligand-receptor pathway alongside TNFSF13/APRIL. Together, these interactions play a crucial role in stimulating B- and T-cell function and regulating humoral immunity. Notably, a third B-cell-specific receptor, BAFFR/BR3, is involved in promoting the survival of mature B-cells and facilitating the B-cell response. This intricate network underscores the significance of BAFF/TNFSF13B in orchestrating immune responses. Additionally, isoform 2 of BAFF/TNFSF13B appears to exert a regulatory role by inhibiting the secretion and bioactivity of isoform 1. The dynamic interplay between these isoforms further contributes to the nuanced control of BAFF/TNFSF13B-mediated immune processes.

Verified Bioactivity

1. Immobilized Human BAFF Trimer, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of ≤0.14 μg/mL determined by ELISA.
2. Immobilized Human BAFF Trimer, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of ≤8 ng/mL determined by ELISA.
3. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μl/well) on the plate. Dose response curve for Human BAFFR, hFc Tag with the EC50 of ≤ 60 ng/mL determined by ELISA.
4. Loaded BAFF/TNFSF13B Trimer Protein, Human on ProA biosensor, can bind Tabalumab (HY-P99220) with an affinity constant of 2.039E-10 M as determined in BLI assay.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded BAFF/TNFSF13B Trimer Protein, Human on ProA biosensor, can bind Tabalumab (HY-P99220) with an affinity constant of 2.039E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-8*His;N-Flag
  • Accession

    Q9Y275-1 (T141-L285)

  • Gene ID
    10673  [NCBI]
  • Molecular Construction
    • N-term
    • 8*His-Flag
    • BAFF (T141-L285)
      Accession # Q9Y275-1
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    TNFSF13B; Dendritic Cell-Derived TNF-Like Molecule; Prev. TNFSF20; Tumor Necrosis Factor Ligand 7A; TALL-1; ZTNF4; TALL1; Tumor Necrosis Factor (Ligand) Superfamily, Member 20; BAFF; TNF- And APOL-Related Leukocyte Expressed Ligand 1; BLYS; Tumor Necrosis

  • AA Sequence

    TVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

  • Predicted Molecular Mass

    52.4 kDa

  • Molecular Weight

    Approximately 53-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Loaded BAFF/TNFSF13B Trimer Protein, Human on ProA biosensor, can bind Tabalumab (HY-P99220) with an affinity constant of 2.039E-10 M as determined in BLI assay.

  • BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) Product Image
    1/16
  • Experimental Validation Results for BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag)
  • BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag) Product Image