Tabalumab
Based on 1 Customer Validation
Tabalumab (LY2127399) is a human anti-BAFF (B-cell activating factor) monoclonal antibody (IgG4 type) with neutralising activity against membrane bound and soluble BAFF. Tabalumab can be used in studies of autoimmune diseases such as rheumatoid arthritis, renal failure and systemic lupus erythematosus.
For research use only. We do not sell to patients.
- Purity: 99%
- CAS No.: 1143503-67-6
- Molecular Weight:145.97 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Human IgG4 kappa
Human
TNFSF13B/BAFF/CD257
Tabalumab (1-100 ng/mL; 44 h) neutralizes soluble and membrane-bound BaFF both in T1165.17 cells and (1 μg/mL; 3 days) CD19+ B-cells[1].
Tabalumab (50 μg/mL; 15 min) inhibits BaFF binding to BR3, TacI, and BcMa in HEK293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:T1165.17 cells
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Concentration:1-100 ng/mL
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Incubation Time:44 h
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Result:Neutralizeed tabalumab neutralization of soluble human, cynomolgus monkey, or rabbit BAFF with IC50 values of 104, 143, and 176 pM in T1165.17 cells, respectively.
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Cell Line:CD19+ B-cells
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Concentration:1 µg/mL
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Incubation Time:3 days
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Result:Demonstrated the ability to neutralize the membrane-bound form of BAFF compared with the soluble form of BAFF.
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Cell Line:HEK293 cells (transfected with either BR3, TACI, or BCMA)
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Concentration:50 µg/mL
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Incubation Time:15 min (pre-treat)
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Result:Prevented the binding of BAFF to BR3, TACI, or BCMA on the cell surface.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Human BAFF Tg mice[1].
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Dosage:500 µg/rat
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Administration:Subcutaneous injection, single.
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Result:Significantly decreased the splenic B-cell count between day 4 and 8.
Decreased levels of p52, which returned to near the BAFF Tg level at Day 23.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG4 kappa
ELISA, FACS, Functional assay
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Loaded BAFF/TNFSF13B Trimer Protein, Human (HEK293, His-Flag, HY-P78384) on ProA biosensor, can bind Tabalumab with an affinity constant of 4.080E-10 M as determined in BLI assay. -
Flow cytometric analysis of 1.5×106 THP-1 cells labeling Tabalumab (HY-P99220, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 (S228P) kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 1143503-67-6
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Appearance Liquid
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Molecular Weight 145.97 kDa
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Color Colorless to light yellow
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SMILES
[Tabalumab]
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Synonyms
LY2127399
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Shipping
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (260 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)