BAG-3 Protein, Mouse (P.pastoris, His)
The BAG-3 protein is a co-chaperone of HSP70 and HSC70, acting as a nucleotide exchange factor to promote the release of ADP from these partners, thus triggering substrate release. BAG-3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived BAG-3 protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Mouse
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The BAG-3 protein is a co-chaperone of HSP70 and HSC70, acting as a nucleotide exchange factor to promote the release of ADP from these partners, thus triggering substrate release. BAG-3 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived BAG-3 protein, expressed by P. pastoris , with N-His labeled tag.
Background
The BAG-3 protein functions as a co-chaperone alongside HSP70 and HSC70 chaperone proteins, playing a pivotal role as a nucleotide-exchange factor (NEF). Its NEF activity facilitates the release of ADP from HSP70 and HSC70, triggering the liberation of client/substrate proteins. The nucleotide release is mediated through its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70, while substrate release is facilitated by its interaction with the substrate-binding domain (SBD) of HSPA8/HSC70. Beyond its role in chaperone activity, BAG-3 exhibits anti-apoptotic activity and is involved in the nucleocytoplasmic transport of HSF1. It binds to the ATPase domain of HSP70/HSC70 chaperones, interacts with BCL2, phospholipase C-gamma proteins, and DNAJB6. Furthermore, BAG-3 engages in interactions with HSF1, HSPA8, HSPA1A, and HSPA1B, and binds to SYNPO2 via its WW domain 1 and SYNPO2's PPPY motif, underscoring its versatile involvement in cellular processes and protein-protein interactions.
Technical Parameters
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Species Mouse
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Source P. pastoris
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Tag N-His
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Accession
Q9JLV1 (S2-P577)
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Molecular Construction
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N-term
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His
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BAG-3 (S2-P577)
Accession # Q9JLV1 -
C-term
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Protein Length
Full Length
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Synonyms
BAG3; BIS; BAG Cochaperone 3; Bcl-2-Binding Protein BIS Transcript 2; BAG Family Molecular Chaperone Regulator 3; BCL2-Binding Athanogene 3; Bcl-2-Binding Protein Bis; CAIR-1; Bcl-2-Associated Athanogene 3; CMD1HH; BCL2 Associated Athanogene 3; CMT2JJ; Do
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AA Sequence
SAATQSPMMQMASGNGASDRDPLPPGWEIKIDPQTGWPFFVDHNSRTTTWNDPRVPPEGPKDTASSANGPSRDGSRLLPIREGHPIYPQLRPGYIPIPVLHEGSENRQPHLFHAYSQPGVQRFRTEAAAATPQRSQSPLRGGMTEAAQTDKQCGQMPATATTAAAQPPTAHGPERSQSPAASDCSSSSSSASLPSSGRSSLGSHQLPRGYIPIPVIHEQNITRPAAQPSFHQAQKTHYPAQQGEYQPQQPVYHKIQGDDWEPRPLRAASPFRSPVRGASSREGSPARSGTPVHCPSPIRVHTVVDRPQPMTHREPPPVTQPENKPESKPGPAGPDLPPGHIPIQVIRREADSKPVSQKSPPPAEKVEVKVSSAPIPCPSPSPAPSAVPSPPKNVAAEQKAAPSPAPAEPAAPKSGEAETPPKHPGVLKVEAILEKVQGLEQAVDSFEGKKTDKKYLMIEEYLTKELLALDSVDPEGRADVRQARRDGVRKVQTILEKLEQKAIDVPGQVQVYELQPSNLEAEQPLQEIMGAVVADKDKKGPENKDPQTESQQLEAKAATPPNPSNPADSAGNLVAP
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Predicted Molecular Mass
63.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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