Butyrylcholinesterase/BCHE Protein, Human (HEK293, His)
Based on 1 Customer Validation
Butyrylcholinesterase/BCHE Protein, Human (HEK293, His) is a recombinant human butyrylcholinesterase (BuChE) produced in HEK293 cells, with His tag. BuChE, one of cholinesterases, is a serine hydrolase closely related to acetylcholinesterase.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Butyrylcholinesterase/BCHE Protein, Human (HEK293, His) is a recombinant human butyrylcholinesterase (BuChE) produced in HEK293 cells, with His tag. BuChE, one of cholinesterases, is a serine hydrolase closely related to acetylcholinesterase[1].
Background
Butyrylcholinesterase is a serine hydrolase closely related to acetylcholinesterase (AChE). Both enzymes have similar three-dimensional-structure. Though their catalytic mechanisms are similar, they display different substrate specificity and inhibitor sensitivity. BuChE is present in plasma and numerous vertebrate tissues. Unlike AChE that plays a key role in terminating the action of acetylcholine in cholinergic system, no direct involvement of BuChE in cholinergic system has been demonstrated. However, BuChE may be a surrogate for AChE in case of deficiency of this enzyme[1].
Verified Bioactivity
Measured by its ability to cleave Butyrylthiocholine. The specific activity is 114442 pmoles/min/μg.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-6*His
-
Accession
P06276 (E29-L602)
-
Molecular Construction
-
N-term
-
BCHE (E29-L602)
Accession # P06276 -
6*His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
BCHE; Pseudocholinesterase; Prev. CHE1; Cholinesterase 1; Prev. CHE2; Cholinesterase; E1; Butyrylcholinesterase, Isoform CRA_a; Acylcholine Acylhydrolase; Carboxylic Ester Hydrolase; Cholinesterase (Serum) 2; BCHE Protein; Butyrylcholine Esterase; BCHED; Butyrylcholinesterase; Choline Esterase II
-
AA Sequence
EDDIIIATKNGKVRGMNLTVFGGTVTAFLGIPYAQPPLGRLRFKKPQSLTKWSDIWNATKYANSCCQNIDQSFPGFHGSEMWNPNTDLSEDCLYLNVWIPAPKPKNATVLIWIYGGGFQTGTSSLHVYDGKFLARVERVIVVSMNYRVGALGFLALPGNPEAPGNMGLFDQQLALQWVQKNIAAFGGNPKSVTLFGESAGAASVSLHLLSPGSHSLFTRAILQSGSFNAPWAVTSLYEARNRTLNLAKLTGCSRENETEIIKCLRNKDPQEILLNEAFVVPYGTPLSVNFGPTVDGDFLTDMPDILLELGQFKKTQILVGVNKDEGTAFLVYGAPGFSKDNNSIITRKEFQEGLKIFFPGVSEFGKESILFHYTDWVDDQRPENYREALGDVVGDYNFICPALEFTKKFSEWGNNAFFYYFEHRSSKLPWPEWMGVMHGYEIEFVFGLPLERRDNYTKAEEILSRSIVKRWANFAKYGNPNETQNNSTSWPVFKSTEQKYLTLNTESTRIMTKLRAQQCRFWTSFFPKVLEMTGNIDEAEWEWKAGFHRWNNYMMDWKNQFNDYTSKKESCVGL
-
Predicted Molecular Mass
67 kDa
-
Molecular Weight
Approximately 70-110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of 50mM Tris, 150mM Nacl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping with dry ice.
Documentation
-
Data Sheet (265 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
References
[1]. Patrick Masson, et al. Structure, activities and biomedical applications of human butyrylcholinesterase. Protein Pept Lett. 2009;16(10):1215-24. [Content Brief]
[2]. Patrick Masson, et al. Butyrylcholinesterase for protection from organophosphorus poisons; catalytic complexities and hysteretic behavior. Arch Biochem Biophys. 2010 Feb 15; 494(2): 107. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
