Copy Link Print Information

Cadherin-13 Protein, Rat (HEK293)

Customer Review

Based on 1 Customer Validation

Cadherin-13 Protein is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13 Protein, Rat (HEK293) is the recombinant rat-derived Cadherin-13 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cadherin-13 Protein, Rat (HEK293)
  • Experimental Validation Results for Cadherin-13 Protein, Rat (HEK293)
  • Cadherin-13 Protein, Rat (HEK293) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Cadherin-13 Protein is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13 Protein, Rat (HEK293) is the recombinant rat-derived Cadherin-13 protein, expressed by HEK293 , with tag free.

Background

Cadherin-13 is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. And it exhibits a preference for homophilic interactions, fostering connections between neighboring cells and potentially playing a role in cell type sorting. Cadherin-13 may act as negative regulators of neural cell growth. Cadherin-13 is highly expressed in nervous and cardiovascular system (in endothelial and smooth muscle cells). Thereby, it may facilitate progression of proliferative vascular disorders such as atherosclerosis, restenosis and tumor angiogenesis. Moreover, it may act rather as a signalling receptor participating in recognition of the environment and regulation of cell motility, proliferation and phenotype. Cadherin-13-dependent activation of PI3K/Akt/GSK3β signalling axis protects endothelial cells from oxidative stress-induced apoptosis.

Verified Bioactivity

Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag Tag Free
  • Accession

    Q8R490 (A22-A692)

  • Gene ID
    192248  [NCBI]
  • Molecular Construction
    • N-term
    • Cadherin-13 (A22-A692)
      Accession # Q8R490
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CDH13; Cadherin-13; Cadherin 13; T-Cad; CDHH; P105; T-Cadherin; Cadherin 13, H-Cadherin (Heart); H-Cadherin (Heart); Truncated Cadherin; Heart Cadherin; H-Cadherin

  • AA Sequence

    AGDLDCTPGFQQKVLHIHQPAEFIEDQPVLNLTFSDCKGNKKLHYEVSSPYFKVNSDGTLVAVRNITAVGRTLFVHARTPHAEDMAELVIVGGKDIQGSLQDIFKFARTSPVPRQKRSIVVSPILIPENQRQPFPRDVGKVVDSDRPEGSKFRLTGKGVDQDPKGTFRINENTGSVSVTRTLDRETIATYQLFVETTDASGRTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIVIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPATGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTKQENISVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLSINPINGTVDTTAVLDRESPFVHNSVYTALFLAIDSGNPPATGTGTLMITLEDVNDNAPVIYPTVAEVCDDARNLSVVILGASDKDLHPNTDPFKFEIHKQTVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCKNSKVDCNAA

  • Molecular Weight

    Approximately 84-96 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Philippova M, et al. A guide and guard: the many faces of T-cadherin. Cell Signal. 2009 Jul;21(7):1035-44. [Content Brief]

[2]. Andreeva AV, et al. Cadherin 13 in cancer. Genes Chromosomes Cancer. 2010 Sep;49(9):775-90. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to inhibit the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 4.702 μg/mL, corresponding to a specific activity is 212.67 units/mg

  • Cadherin-13 Protein, Rat (HEK293) Product Image
    1/16
  • Experimental Validation Results for Cadherin-13 Protein, Rat (HEK293)
  • Experimental Validation Results for Cadherin-13 Protein, Rat (HEK293)
  • Cadherin-13 Protein, Rat (HEK293) Product Image