1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Immunoglobulin-like Cell Adhesion Molecules
  5. Cell Adhesion Molecule 1/TSLC-1
  6. CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His)

CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His)

Cat. No.: HY-P70085
COA Handling Instructions

CADM1/IGSF4A proteins coordinate Ca(2+)-independent homophilic and heterophilic cell adhesion with CADM3 and NECTIN3. Interaction with CRTAM enhances NK cell cytotoxicity and CD8+ T cell function. CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His) is the recombinant mouse-derived CADM1/IGSF4A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His) is 341 a.a., with molecular weight of 60-100 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
10 μg $152 In-stock
50 μg $400 In-stock
100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CADM1/IGSF4A proteins coordinate Ca(2+)-independent homophilic and heterophilic cell adhesion with CADM3 and NECTIN3. Interaction with CRTAM enhances NK cell cytotoxicity and CD8+ T cell function. CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His) is the recombinant mouse-derived CADM1/IGSF4A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His) is 341 a.a., with molecular weight of 60-100 kDa.

Background

CADM1/IGSF4A Protein orchestrates homophilic cell-cell adhesion in a Ca(2+)-independent manner, as substantiated by multiple studies. Furthermore, it engages in heterophilic cell-cell adhesion with CADM3 and NECTIN3, independent of calcium levels. Its interaction with CRTAM not only fosters natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ T-cells but also facilitates NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, CADM1 may play a role in attachment to and communication with nerves, being indispensable for the development and survival of mast cells in vivo. The protein's involvement extends to regulating the retention of activated CD8+ T-cells within the draining lymph node and influencing the intestinal retention of intraepithelial CD4+ CD8+ T-cells. Additionally, CADM1 acts as a synaptic cell adhesion molecule, contributing to dendritic spine formation and synapse assembly, while potentially influencing neuronal migration, axon growth, pathfinding, and fasciculation. Its diverse roles also encompass participation in spermatogenesis, including the adhesion of spermatocytes and spermatids to Sertoli cells and their differentiation into mature spermatozoa. The intricate network of CADM1 interactions involves homodimerization, association with FARP1, CRTAM, EPB41L3/DAL1, MPP2, MPP3, and PALS2, providing a comprehensive framework for understanding its multifaceted contributions.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

Q8R5M8 (Q48-H388)

Gene ID

54725  [NCBI]

Molecular Construction
N-term
CADM1 (Q48-H388)
Accession # Q8R5M8
6*His
C-term
Synonyms
rMuCell adhesion molecule 1/CADM1, His; Cell adhesion molecule 1; Immunoglobulin superfamily member 4; IgSF4; Nectin-like protein 2; NECL-2; Synaptic cell adhesion molecule; SynCAM; Tumor suppressor in lung cancer 1; TSLC-1
AA Sequence

QNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNKELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDAFELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDTTATTEPAVHDSRAGEEGTIGAVDH

Molecular Weight

60-100 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CADM1/IGSF4A Protein, Mouse (341a.a, HEK293, His)
Cat. No.:
HY-P70085
Quantity:
MCE Japan Authorized Agent: