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Carbonic Anhydrase 2 Protein, Human (HEK293, His)

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Carbonic Anhydrase 2 (CA2) catalyzes the reversible hydration of carbon dioxide. Mutations in CA2 are linked to osteopetrosis and renal tubular acidosis. The gene exhibits biased expression in various tissues, notably in the stomach and colon. Two transcript variants, encoding different isoforms, have been identified. Carbonic Anhydrase 2 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Carbonic Anhydrase 2 Protein, Human (HEK293, His)
  • Carbonic Anhydrase 2 Protein, Human (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Carbonic Anhydrase 2 (CA2) catalyzes the reversible hydration of carbon dioxide. Mutations in CA2 are linked to osteopetrosis and renal tubular acidosis. The gene exhibits biased expression in various tissues, notably in the stomach and colon. Two transcript variants, encoding different isoforms, have been identified. Carbonic Anhydrase 2 Protein, Human (HEK293, His) is the recombinant human-derived Carbonic Anhydrase 2 protein, expressed by HEK293 , with C-His labeled tag.

Background

Carbonic Anhydrase 2 Protein is a member of the carbonic anhydrase isozyme family, responsible for catalyzing the reversible hydration of carbon dioxide. Dysregulation of this enzyme is linked to conditions such as osteopetrosis and renal tubular acidosis. Two transcript variants encoding distinct isoforms have been identified. In addition to its fundamental role in carbon dioxide metabolism, the protein exhibits biased expression in various tissues, with notable levels in the stomach and colon, as well as eight other tissues. This tissue-specific expression profile suggests its potential involvement in specialized physiological processes beyond its well-established functions in acid-base balance.

Verified Bioactivity

Measured by its esterase activity. The specific activity is 1454.8 pmol/min/µg, as measured under the described conditions.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Assay Procedure

Materials
Assay buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5
Carbonic Anhydrase 2 Protein, Human (HEK293, His) (HY-P78746)
Substrate: 4-NPA, diluted to 100 mM in DMSO

Procedure
1. Standard curve preparation: Prepare 4-nitrophenol standards at concentrations of 5×108, 2.5×108, 1.25×108, 0.625×108, 0.3125×108, 0.15265×108, and 0 pmo/mL. Measure the OD400 for each standard concentration. Plot a standard curve with the amount of standard substance (pmol) as the ordinate and OD400 as the abscissa.
2. Dilute Human CA2 to 20 μg/mL in assay buffer.
3. Dilute the substrate to 2 mM in assay buffer.
4. Add 50 μL of 20 μg/mL Human CA2 to a 96-well plate, and initiate the reaction by adding 50 μL of 2 mM substrate to the wells.
5. Blank group: 50 μL assay buffer and 50 μL of 2 mM substrate.
6. Zero the microplate reader using the OD value of the blank group, and read the absorbance at 400 nm in kinetic mode for 5 min.
7. Calculate the specific activity:

     Specific Activity (pmol/min/μg) =

Adjusted Vmax* (OD/min) x Conversion Factor ** (pmol/OD)
amount of enzyme (μg)

*Adjusted for Substrate Blank
**Derived using calibration standard

Per Well:
Human CA2: 1 μg
Substrate: 1 mM

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    NP_000058.1 (M1-K260)

  • Gene ID
    760  [NCBI]
  • Molecular Construction
    • N-term
    • CA2 (M1-K260)
      Accession # NP_000058.1
    • His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    CA2; CAC; Carbonic Anhydrase 2; Epididymis Secretory Protein Li 282; Carbonic Anhydrase II; Epididymis Luminal Protein 76; CA-II; Carbonic Anhydrase B; Carbonate Dehydratase II; Carbonic Dehydratase; Cyanamide Hydratase CA2; Carbonic Anhydrase; CAII; HEL-

  • AA Sequence

    MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDTHTAKYDPSLKPLSVSYDQATSLRILNNGHAFNVEFDDSQDKAVLKGGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNTKYGDFGKAVQQPDGLAVLGIFLKVGSAKPGLQKVVDVLDSIKTKGKSADFTNFDPRGLLPESLDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGEGEPEELMVDNWRPAQPLKNRQIKASFK

  • Molecular Weight

    Approximately 31-33 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HC1, 0.5 M NaCl, 6% trehalose, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Carbonic Anhydrase 2 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Carbonic Anhydrase 2 Protein, Human (HEK293, His)
  • Carbonic Anhydrase 2 Protein, Human (HEK293, His) Product Image