Carboxypeptidase M/CPM Protein, Human (HEK293, His)

Carboxypeptidase M Protein, Human (HEK293, His) is an approximately 55.0 kDa Carboxypeptidase M protein with a His-flag. Carboxypeptidase M is a membrane-bound arginine/lysine carboxypeptidase enzyme belongs to the carboxypeptidases family^[1].

Carboxypeptidase M is a membrane-bound arginine/lysine carboxypeptidase enzyme, cleaves C-terminal basic amino acids with a neutral pH optimum^[1].
  Carboxypeptidase M belongs to the carboxypeptidases family. Carboxypeptidase M is widely distributed in a variety of tissues and cells, and it constitutively expresses in an active form at the surface of specialized cells and tissues in the human body^[2].
Carboxypeptidase M plays an important role in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing^[3].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P14384 (L18-D421)

1368  [NCBI]

LDFNYHRQEGMEAFLKTVAQNYSSVTHLHSIGKSVKGRNLWVLVVGRFPKEHRIGIPEFKYVANMHGDETVGRELLLHLIDYLVTSDGKDPEITNLINSTRIHIMPSMNPDGFEAVKKPDCYYSIGRENYNQYDLNRNFPDAFEYNNVSRQPETVAVMKWLKTETFVLSANLHGGALVASYPFDNGVQATGALYSRSLTPDDDVFQYLAHTYASRNPNMKKGDECKNKMNFPNGVTNGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPSFWNNNKASLIEYIKQVHLGVKGQVFDQNGNPLPNVIVEVQDRKHICPYRTNKYGEYYLLLLPGSYIINVTVPGHDPHITKVIIPEKSQNFSALKKDILLPFQGQLDSIPVSNPSCPMIPLYRNLPDHHHHHHH

Approximately 55.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filter solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM ZnCl₂, pH7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. A Nagae, et al. Carboxypeptidase M in brain and peripheral nerves. J Neurochem  [Content Brief]

  [2]. Kathleen Deiteren, et al. Carboxypeptidase M: Multiple alliances and unknown partners. Clin Chim Acta. 2009 Jan;399(1-2):24-39.  [Content Brief]

  [3]. David Reverter, Crystal structure of human carboxypeptidase M, a membrane-bound enzyme that regulates peptide hormone activity. J Mol Biol  [Content Brief]