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Cas9 Protein, S. pyogenes (sf9, NLS, His)

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The Cas9 protein is part of an immune system that defends against genetic elements.It processes RNA and aids in cleaving DNA targets.Protein and guide RNAs are necessary for its function.The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein, Streptococcus pyogenes M1 (sf9, NLS, His) is the recombinant Cas9 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Cas9 Protein, S. pyogenes (sf9, NLS, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Cas9 protein is part of an immune system that defends against genetic elements.It processes RNA and aids in cleaving DNA targets.Protein and guide RNAs are necessary for its function.The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein, Streptococcus pyogenes M1 (sf9, NLS, His) is the recombinant Cas9 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

Cas9, a revolutionary molecular tool, constitutes an adaptive immune system that defends against mobile genetic elements like viruses, transposable elements, and conjugative plasmids. The process involves transcription and processing of crRNA, wherein, for type II gene editing systems, the proper processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and the pivotal Cas9 protein. The tracrRNA guides ribonuclease 3 in processing pre-crRNA, with Cas9 stabilizing the pre-crRNA:tracrRNA interaction. In subsequent steps, Cas9/crRNA/tracrRNA complex acts as an endonuclease, cleaving the target DNA with complementary spacer sequences. Cas9 is inactive without the guidance of two specific guide RNAs (gRNA), emphasizing the necessity of both gRNAs for DNA binding and nuclease activity. This sophisticated system confers immunity against invading genetic elements through a mechanism known as gene editing interference.

Verified Bioactivity

1. 1 μg = 5 U ,1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃ .
2. Cas9 Protein achieves ≥ 90% substrate cleavage measured by an in vitro DNA cleavage assay.

Technical Parameters

  • Species Others
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    WP_010922251 (M1-D1368)

  • Gene ID
    /
  • Protein Length

    Full Length

  • Synonyms

    SPy_1046; csn1; cas9; SpyCas9; SpCas9; EC:3.1.-.- ; CRISPR-associated endonuclease Cas9/Csn1

  • AA Sequence

    MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD

  • Predicted Molecular Mass

    163 kDa

  • Molecular Weight

    Approximately 140 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 20-50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Cas9 Protein, S. pyogenes (sf9, NLS, His) Product Image