1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Cathepsin A Protein, Human (HEK293, His, solution)

Cathepsin A Protein, Human (HEK293, His, solution)

Cat. No.: HY-P7745
Handling Instructions

Cathepsin A Protein, Human (HEK293, His, solution) is a 58-60 kDa human cathepsin A protein with a His-flag. Cathepsin A is a multicatalytic enzyme with carboxypeptidase activities[1].

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $165 In-stock
Estimated Time of Arrival: December 31
50 μg $496 In-stock
Estimated Time of Arrival: December 31
100 μg   Get quote  
250 μg   Get quote  

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Cathepsin A Protein, Human (HEK293, His, solution) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK 293

Tag

C-His

Accession

P10619 (S69-Y480)

Gene ID

5476

Synonyms
rHuCathepsin A, His; Lysosomal protective protein; CTSA; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A
AA Sequence

SQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

Molecular Weight

58-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Biological Activity
Data is not available.
Appearance

Solution

Formulation

Supplied as a 0.2 μm filter solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Background

Cathepsin A is synthesized as a zymogen, a single-chain precursor of 54 kDa (after signal peptide cleavage and addition of two N-linked glycans) that self-associates into a homodimer. The precursor cathepsin A has two domains, a core domain (1–182 and 303–452) interrupted by a cap domain insertion (183–302).
Cathepsin A is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities.
Cathepsin A, lysosomal carboxypeptidase are components of the lysosomal multienzyme complex along with beta-galactosidase. Cathepsin A activates Neu1 and protects GAL and Neu1 against the rapid proteolytic degradation.

Documentation
References
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Cathepsin A Protein, Human (HEK293, His, solution)
Cat. No.:
HY-P7745
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