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Cathepsin A Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

Cathepsin A protein is an important protective factor for β-galactosidase and neuraminidase, maintaining their stability and optimizing enzyme activity. Its carboxypeptidase activity expands its functional repertoire and can deamid tachykinins and participate in a variety of biochemical processes. Cathepsin A Protein, Human (HEK293, His) is the recombinant human-derived Cathepsin A, expressed by HEK293, with C-6*His labeled tag. The total length of Cathepsin A Protein, Human (HEK293, His) is 452 a.a..

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Cathepsin A Protein, Human (HEK293, His)
  • Cathepsin A Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Cathepsin A protein is an important protective factor for β-galactosidase and neuraminidase, maintaining their stability and optimizing enzyme activity. Its carboxypeptidase activity expands its functional repertoire and can deamid tachykinins and participate in a variety of biochemical processes. Cathepsin A Protein, Human (HEK293, His) is the recombinant human-derived Cathepsin A, expressed by HEK293, with C-6*His labeled tag. The total length of Cathepsin A Protein, Human (HEK293, His) is 452 a.a..

Background

Cathepsin A Protein emerges as a crucial protective factor essential for the activity of both beta-galactosidase and neuraminidase. Operating in association with these enzymes, Cathepsin A plays a pivotal role in preserving their stability and ensuring optimal enzymatic activity. Beyond its protective function, this protein exhibits carboxypeptidase activity, broadening its functional repertoire. Notably, Cathepsin A demonstrates the ability to deamidate tachykinins, showcasing its involvement in diverse biochemical processes. The multifaceted roles of Cathepsin A underscore its significance in maintaining the integrity and functionality of key enzymatic processes, thereby contributing to cellular homeostasis.

Verified Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate of 20 μM Mca-RPPGFSAFK(Dnp)-OH (HY-P3749) that incubate at room temperature for 5 minutes. The specific activity is 3621.65 pmol/min/ug. (Activation description: The proenzyme needs to be activated by Human Cathepsin L1 (HY-P7754A) for an activated form.)

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P10619-1 (A29-Y480)

  • Gene ID
    5476  [NCBI]
  • Molecular Construction
    • N-term
    • Cathepsin A (A29-Y480)
      Accession # P10619
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CTSA; PPCA; Prev. PPGB; Protective Protein For Beta-Galactosidase; Prev. GSL; Beta-Galactosidase Protective Protein; Lysosomal Protective Protein; Alternative Protein CTSA; Carboxypeptidase C; Beta-Galactosidase 2; Carboxypeptidase Y-Like Kininase; Carbox

  • AA Sequence

    APDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

  • Predicted Molecular Mass

    51.4 kDa

  • Molecular Weight

    Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Cathepsin A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Cathepsin A Protein, Human (HEK293, His)
  • Cathepsin A Protein, Human (HEK293, His) Product Image