Cathepsin A Protein, Human (HEK293, His)

Cathepsin A protein is an important protective factor for β-galactosidase and neuraminidase, maintaining their stability and optimizing enzyme activity. Its carboxypeptidase activity expands its functional repertoire and can deamid tachykinins and participate in a variety of biochemical processes. Cathepsin A Protein, Human (HEK293, His) is the recombinant human-derived Cathepsin A, expressed by HEK293, with C-6*His labeled tag. The total length of Cathepsin A Protein, Human (HEK293, His) is 452 a.a..

Cathepsin A Protein emerges as a crucial protective factor essential for the activity of both beta-galactosidase and neuraminidase. Operating in association with these enzymes, Cathepsin A plays a pivotal role in preserving their stability and ensuring optimal enzymatic activity. Beyond its protective function, this protein exhibits carboxypeptidase activity, broadening its functional repertoire. Notably, Cathepsin A demonstrates the ability to deamidate tachykinins, showcasing its involvement in diverse biochemical processes. The multifaceted roles of Cathepsin A underscore its significance in maintaining the integrity and functionality of key enzymatic processes, thereby contributing to cellular homeostasis.

Measured by its ability to cleave a colorimetric peptide substrate of 20 μM Mca-RPPGFSAFK(Dnp)-OH (HY-P3749) that incubate at room temperature for 5 minutes. The specific activity is 3621.65 pmol/min/ug. (Activation description: The proenzyme needs to be activated by Human Cathepsin L1 (HY-P7754A) for an activated form.)

Human

HEK293

C-6*His

P10619-1 (A29-Y480)

5476  [NCBI]

Full Length of Isoform-1 Mature Protein

APDQDEIQRLPGLAKQPSFRQYSGYLKGSGSKHLHYWFVESQKDPENSPVVLWLNGGPGCSSLDGLLTEHGPFLVQPDGVTLEYNPYSWNLIANVLYLESPAGVGFSYSDDKFYATNDTEVAQSNFEALQDFFRLFPEYKNNKLFLTGESYAGIYIPTLAVLVMQDPSMNLQGLAVGNGLSSYEQNDNSLVYFAYYHGLLGNRLWSSLQTHCCSQNKCNFYDNKDLECVTNLQEVARIVGNSGLNIYNLYAPCAGGVPSHFRYEKDTVVVQDLGNIFTRLPLKRMWHQALLRSGDKVRMDPPCTNTTAASTYLNNPYVRKALNIPEQLPQWDMCNFLVNLQYRRLYRSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGDEWFVDSLNQKMEVQRRPWLVKYGDSGEQIAGFVKEFSHIAFLTIKGAGHMVPTDKPLAAFTMFSRFLNKQPY

Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.