CBLC Protein, Human (sf9, His-GST)
CBLC protein is an E3 ubiquitin-protein ligase that plays a crucial regulatory role in the ubiquitin-proteasome system by accepting ubiquitin from specific E2 enzymes and promoting substrate degradation. It forms a functional complex with UB2D1, UB2D2 and UB2D3. CBLC Protein, Human (sf9, His-GST) is the recombinant human-derived CBLC protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CBLC protein is an E3 ubiquitin-protein ligase that plays a crucial regulatory role in the ubiquitin-proteasome system by accepting ubiquitin from specific E2 enzymes and promoting substrate degradation. It forms a functional complex with UB2D1, UB2D2 and UB2D3. CBLC Protein, Human (sf9, His-GST) is the recombinant human-derived CBLC protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
CBLC protein functions as an E3 ubiquitin-protein ligase, playing a crucial role in the ubiquitin-proteasome system by accepting ubiquitin from specific E2 ubiquitin-conjugating enzymes and transferring it to substrates, thereby promoting their degradation. It forms a functional complex with E2 ubiquitin-protein ligases UB2D1, UB2D2, and UB2D3. CBLC acts as a regulator in EGFR-mediated signal transduction, where upon EGF activation, it ubiquitinates EGFR, leading to its degradation. Isoform 1, in contrast to isoform 2, inhibits MAPK1 activation stimulated by EGF. Additionally, CBLC promotes the ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP, CBLC serves as a regulatory checkpoint for Ret signaling by modulating the rate of RET degradation following ligand activation. The partnership with CD2AP transforms CBLC from an inhibitor to a promoter of RET degradation, limiting the potency of GDNF on neuronal survival in the process.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
AAH28915.1 (M1-A474)
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Molecular Construction
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N-term
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His-GST
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CBLC (M1-A474)
Accession # AAH28915.1 -
C-term
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Protein Length
Full Length
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Synonyms
CBLC; E3 Ubiquitin-Protein Ligase CBL-C; Cbl Proto-Oncogene C; Signal Transduction Protein CBL-C; RNF57; SH3-Binding Protein CBL-3; CBL-SL; SH3-Binding Protein CBL-C; CBL-3; RING Finger Protein 57; Cas-Br-M (Murine) Ecotropic Retroviral Transforming Seque
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AA Sequence
MALAVAPWGRQWEEARALGRAVRMLQRLEEQCVDPRLSVSPPSLRDLLPRTAQLLREVAHSRRAAGGGGPGGPGGSGDFLLIYLANLEAKSRQVAALLPPRGRRSANDELFRAGSRLRRQLAKLAIIFSHMHAELHALFPGGKYCGHMYQLTKAPAHTFWRESCGARCVLPWAEFESLLGTCHPVEPGCTALALRTTIDLTCSGHVSIFEFDVFTRLFQPWPTLLKNWQLLAVNHPGYMAFLTYDEVQERLQACRDKPGSYIFRPSCTRLGQWAIGYVSSDGSILQTIPANKPLSQVLLEGQKDGFYLYPDGKTHNPDLTELGQAEPQQRIHVSEEQLQLYWAMDSTFELCKICAESNKDVKIEPCGHLLCSCCLAAWQHSDSQTCPFCRCEIKGWEAVSIYQFHGQATAEDPGNSSDQEGRELELGQVPLSAPPLPPRPDLPPRKPRNAQPKVRLLKGNSPPAALGPQDPAPA
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Predicted Molecular Mass
80.2 kDa
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Molecular Weight
Approximately 66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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