1. Recombinant Proteins
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E3 Ligases
  5. CBL-C Proteins
  6. CBLC Protein, Human (sf9, His-GST)

CBLC Protein, Human (sf9, His-GST)

Cat. No.: HY-P74343
Handling Instructions

CBLC Protein, an E3 ubiquitin-protein ligase, crucially regulates the ubiquitin-proteasome system by accepting ubiquitin from specific E2 enzymes and promoting substrate degradation. It forms a functional complex with UB2D1, UB2D2, and UB2D3. In EGFR-mediated signaling, CBLC ubiquitinates EGFR upon EGF activation, leading to its degradation. Isoform 1 inhibits EGF-stimulated MAPK1 activation, while CBLC, along with CD2AP, regulates RET degradation, transforming from an inhibitor to a promoter, limiting GDNF's potency on neuronal survival during Ret signaling. CBLC Protein, Human (sf9, His-GST) is the recombinant human-derived CBLC protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. The total length of CBLC Protein, Human (sf9, His-GST) is 474 a.a., with molecular weight of ~66 kDa.

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Description

CBLC Protein, an E3 ubiquitin-protein ligase, crucially regulates the ubiquitin-proteasome system by accepting ubiquitin from specific E2 enzymes and promoting substrate degradation. It forms a functional complex with UB2D1, UB2D2, and UB2D3. In EGFR-mediated signaling, CBLC ubiquitinates EGFR upon EGF activation, leading to its degradation. Isoform 1 inhibits EGF-stimulated MAPK1 activation, while CBLC, along with CD2AP, regulates RET degradation, transforming from an inhibitor to a promoter, limiting GDNF's potency on neuronal survival during Ret signaling. CBLC Protein, Human (sf9, His-GST) is the recombinant human-derived CBLC protein, expressed by Sf9 insect cells, with N-His, N-GST labeled tag. The total length of CBLC Protein, Human (sf9, His-GST) is 474 a.a., with molecular weight of ~66 kDa.

Background

CBLC protein functions as an E3 ubiquitin-protein ligase, playing a crucial role in the ubiquitin-proteasome system by accepting ubiquitin from specific E2 ubiquitin-conjugating enzymes and transferring it to substrates, thereby promoting their degradation. It forms a functional complex with E2 ubiquitin-protein ligases UB2D1, UB2D2, and UB2D3. CBLC acts as a regulator in EGFR-mediated signal transduction, where upon EGF activation, it ubiquitinates EGFR, leading to its degradation. Isoform 1, in contrast to isoform 2, inhibits MAPK1 activation stimulated by EGF. Additionally, CBLC promotes the ubiquitination of SRC phosphorylated at 'Tyr-419'. In collaboration with CD2AP, CBLC serves as a regulatory checkpoint for Ret signaling by modulating the rate of RET degradation following ligand activation. The partnership with CD2AP transforms CBLC from an inhibitor to a promoter of RET degradation, limiting the potency of GDNF on neuronal survival in the process.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q9ULV8 (M1-A474)

Gene ID
Molecular Construction
N-term
His-GST
CBLC (M1-A474)
Accession # Q9ULV8
C-term
Synonyms
E3 ubiquitin-protein ligase CBL-C; CBLC; CBL3; RNF57
Molecular Weight

Approximately 66 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CBLC Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CBLC Protein, Human (sf9, His-GST)
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HY-P74343
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