CD146/MCAM Protein, Cynomolgus (HEK293, His)
The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of CD146/MCAM Protein, Cynomolgus (HEK293, His) is 536 a.a., with molecular weight of 80-110 kDa.
- Species: Cynomolgus
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The CD146/MCAM protein is a multifunctional adhesion regulator that plays a crucial role in maintaining endothelial monolayer adhesion in vascular tissue. Its expression is associated with promoting interactions between melanoma cells and vascular components, potentially enhancing hematogenous spread of tumors. CD146/MCAM Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag. The total length of CD146/MCAM Protein, Cynomolgus (HEK293, His) is 536 a.a., with molecular weight of 80-110 kDa.
Background
CD146/MCAM, a versatile protein, assumes a crucial role in cell adhesion and the cohesion of the endothelial monolayer at intercellular junctions within vascular tissue. Its expression holds significance in facilitating interactions between melanoma cells and cellular components of the vascular system, potentially augmenting the hematogeneous spread of tumors. Beyond its involvement in tumor biology, CD146/MCAM is postulated to function as an adhesion molecule during embryonic development, particularly in neural crest cells. Acting as a surface receptor, it triggers tyrosine phosphorylation events involving FYN and PTK2/FAK1, accompanied by a transient increase in intracellular calcium concentration (By similarity).
Technical Parameters
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Species Cynomolgus
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Source HEK293
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Tag C-His
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Accession
A0A5F8A4A1 (V24-G559)
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Gene ID/
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Molecular Construction
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N-term
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MCAM (V24-G559)
Accession # A0A5F8A4A1 -
His
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C-term
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Protein Length
Partial
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Synonyms
MCAM; Melanoma-Associated Antigen MUC18; Melanoma Cell Adhesion Molecule; Cell Surface Glycoprotein MUC18; MUC18; Cell Surface Glycoprotein P1H12; MelCAM; Melanoma-Associated Antigen A32; METCAM; Melanoma Adhesion Molecule; HEMCAM; Gicerin; CD146; CD146 Antigen; S-Endo 1 Endothelial-Associated Antigen
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AA Sequence
VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRIPIFRVRQGQGQSEPGEYQHRLSLQDRGATLALTQVTPHDERIFLCQGKRPRSQEHRIQLRVYKAPEEPNIQVNALGIPVDSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLKSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPAQKEHSGLYECQGLDLDTMISLPSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCKAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAVFGPPWMAFKERKVWVKENMMLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSVLFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESQG
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Molecular Weight
Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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