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CD3 gamma Protein, Human (HEK293, Fc)

Cat. No.: HY-P78753
COA Handling Instructions

CD3 gamma, part of the TCR-CD3 complex on T-lymphocytes, facilitates TCR-mediated signaling and regulates surface expression. APC-induced TCR activation prompts CD3 gamma, along with CD3D, CD3E, and CD3Z, to transmit signals via ITAMs. Phosphorylation by LCK and FYN initiates downstream signaling. CD3 gamma's diL motif guides constitutive TCR cycling. In TCR-CD3 assembly, CD3 gamma shows versatility, forming heterodimers and trimers, highlighting its pivotal role in T-cell activation and receptor dynamics. CD3 gamma Protein, Human (HEK293, Fc) is the recombinant human-derived CD3 gamma protein, expressed by HEK293, with C-hFc labeled tag. The total length of CD3 gamma Protein, Human (HEK293, Fc) is 94 a.a., with molecular weight of 45-55 kDa.

For research use only. We do not sell to patients.

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Description

CD3 gamma, part of the TCR-CD3 complex on T-lymphocytes, facilitates TCR-mediated signaling and regulates surface expression. APC-induced TCR activation prompts CD3 gamma, along with CD3D, CD3E, and CD3Z, to transmit signals via ITAMs. Phosphorylation by LCK and FYN initiates downstream signaling. CD3 gamma's diL motif guides constitutive TCR cycling. In TCR-CD3 assembly, CD3 gamma shows versatility, forming heterodimers and trimers, highlighting its pivotal role in T-cell activation and receptor dynamics. CD3 gamma Protein, Human (HEK293, Fc) is the recombinant human-derived CD3 gamma protein, expressed by HEK293, with C-hFc labeled tag. The total length of CD3 gamma Protein, Human (HEK293, Fc) is 94 a.a., with molecular weight of 45-55 kDa.

Background

CD3 gamma, an integral component of the TCR-CD3 complex on the T-lymphocyte cell surface, is pivotal in the adaptive immune response. As antigen-presenting cells (APCs) activate the T-cell receptor (TCR), CD3 gamma, along with other CD3 chains (CD3D, CD3E, and CD3Z), facilitates the transmission of TCR-mediated signals across the cell membrane. With immunoreceptor tyrosine-based activation motifs (ITAMs) in its cytoplasmic domain, CD3 gamma undergoes phosphorylation by Src family protein tyrosine kinases LCK and FYN upon TCR engagement, activating downstream signaling pathways. Beyond its role in signal transduction, CD3 gamma plays a crucial role in dynamically regulating TCR expression at the cell surface. Constitutive TCR cycling relies on the di-leucine-based (diL) receptor-sorting motif present in CD3 gamma. The TCR-CD3 complex comprises CD3D/CD3E and CD3G/CD3E heterodimers, which preferentially associate with TCRalpha and TCRbeta, forming trimers that interact with CD3Z homodimers to complete the hexameric TCR-CD3 complex. Alternatively, TCRalpha and TCRbeta can be replaced by TCRgamma and TCRdelta, showcasing the versatility of CD3 gamma in TCR assembly and functionality.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

P09693 (Q23-S116)

Gene ID

917  [NCBI]

Molecular Construction
N-term
CD3 gamma (Q23-S116)
Accession # P09693
hFc
C-term
Synonyms
CD3G; CD3-GAMMA; IMD17; T3G; TCR gamma
Molecular Weight

45-55 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD3 gamma Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD3 gamma Protein, Human (HEK293, Fc)
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HY-P78753
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