1. Recombinant Proteins
  2. CD Antigens
  3. T Cell CD Proteins
  4. CD3D-CD3E Heterodimer Protein, Human (HEK293, Fc)

CD3D-CD3E Heterodimer Protein, Human (HEK293, Fc)

Cat. No.: HY-P77610
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
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100 μg Ask For Quote & Lead Time

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CD3D-CD3E Heterodimer Protein, Human (HEK293, Fc) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

HEK 293

Tag

hFc

Accession

P04234 (F22-A105)&P07766 (D23-D126)

Gene ID

915&916

Synonyms
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon
AA Sequence

Molecular Weight

40-50 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC

Biological Activity
Immobilized Human CD3E&CD3D at 0.5 μg/mL (100μL/Well). Dose response curve for Biotinylated Anti-CD3E&CD3D Ab.1 with the EC50 of 31.68 ng/mL determined by ELISA.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
CD3D-CD3E Heterodimer Protein, Human (HEK293, Fc)
Cat. No.:
HY-P77610
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