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CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi)

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Based on 1 Customer Validation

CLEC12A/MICL (C-type lectin domain family 12 member A/myelosuppressive C-type lectin-like receptor) is a cell surface receptor that acts as a modulator of signaling cascades, specifically promoting target MAP kinases Protease. CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CLEC12A/MICL protein, expressed by HEK293 , with C-Avi, N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi)
  • CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CLEC12A/MICL (C-type lectin domain family 12 member A/myelosuppressive C-type lectin-like receptor) is a cell surface receptor that acts as a modulator of signaling cascades, specifically promoting target MAP kinases Protease. CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived CLEC12A/MICL protein, expressed by HEK293 , with C-Avi, N-His labeled tag.

Background

CLEC12A/MICL (C-type lectin domain family 12 member A/Myeloid inhibitory C-type lectin-like receptor) is a cell surface receptor that functions as a regulator of signaling cascades, specifically facilitating the tyrosine phosphorylation of target MAP kinases. Through its interactions with PTPN6 and PTPN11, CLEC12A/MICL is implicated in intricate cellular signaling processes, suggesting its significance in modulating immune responses and potentially serving as a target for therapeutic interventions aimed at manipulating immune system function.

Verified Bioactivity

1. Immobilized Anti-CLEC12A Antibody,hFc Tag at 1ug/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human CLEC12A,His Tag with the EC50 of 0.53ug/ml determined by ELISA.
2. Loaded Tepoditamab (HY-P99390) on AHC2 biosensor, can bind CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.185E-08 M as determined in BLI assay.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Tepoditamab (HY-P99390) on AHC2 biosensor, can bind CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.185E-08 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;N-His
  • Accession

    Q5QGZ9-2 (H65-A265)

  • Gene ID
    160364  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • CLEC12A (H65-A265)
      Accession # Q5QGZ9-2
    • Avi
    • C-term
  • Protein Length

    Extracellular Domain

  • Conjugation

    Biotin

  • Synonyms

    CLEC12A; CLL1; C-Type Lectin Domain Family 12 Member A; C-Type Lectin Domain Family 12, Member A; DCAL-2; C-Type Lectin-Like Molecule-1; CLL-1; C-Type Lectin-Like Molecule 1; MICL; C-Type Lectin Protein CLL-1; Myeloid Inhibitory C-Type Lectin-Like Recepto

  • AA Sequence

    HVTLKIEMKKMNKLQNISEELQRNISLQLMSNMNISNKIRNLSTTLQTIATKLCRELYSKEQEHKCKPCPRRWIWHKDSCYFLSDDVQTWQESKMACAAQNASLLKINNKNALEFIKSQSRSYDYWLGLSPEEDSTRGMRVDNIINSSAWVIRNAPDLNNMYCGYINRLYVQYYHCTYKKRMICEKMANPVQLGSTYFREA

  • Predicted Molecular Mass

    26.4 kDa

  • Molecular Weight

    Approximately 38-50 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Loaded Tepoditamab (HY-P99390) on AHC2 biosensor, can bind CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.185E-08 M as determined in BLI assay.

  • CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
    1/16
  • Experimental Validation Results for CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi)
  • CLEC12A/MICL Protein, Human (Biotinylated, HEK293, His-Avi) Product Image