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  3. Oxidoreductases (EC 1)
  4. COX-2 Protein, Human (sf9, His)

COX-2 Protein, Human (sf9, His)

Cat. No.: HY-P74222
COA Handling Instructions

COX-2 protein is involved in the alternative pathway of prostaglandin biosynthesis. COX-2 Protein, Human (sf9, His) is the recombinant human-derived COX-2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.

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50 μg $475 In-stock
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  • Biological Activity

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Description

COX-2 protein is involved in the alternative pathway of prostaglandin biosynthesis. COX-2 Protein, Human (sf9, His) is the recombinant human-derived COX-2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

COX-2 protein serves as a dual functional enzyme in the biosynthesis pathway of prostanoids, critical mediators in the inflammatory response. Acting as both a cyclooxygenase and peroxidase, COX-2 transforms arachidonate (AA) into the hydroperoxy endoperoxide prostaglandin G2 (PGG2) and further reduces it to the hydroxy endoperoxide prostaglandin H2 (PGH2). This catalytic process involves hydrogen abstraction at carbon 13, followed by molecular oxygen insertion to form the characteristic endoperoxide bridge in prostaglandins. COX-2 extends its enzymatic activity to dihomo-gamma-linoleate (DGLA) and eicosapentaenoate (EPA), generating PGH1 and PGH3 as precursors of 1- and 3-series prostaglandins. Additionally, COX-2 contributes to the alternative prostanoid biosynthesis pathway, converting 2-arachidonoyl lysophospholipids and metabolizing 2-arachidonoyl glycerol. The enzyme also plays a role in the generation of resolution phase interaction products, contributing to the synthesis of specialized pro-resolving mediators during inflammatory processes, such as resolvins. This multifaceted functionality highlights the pivotal role of COX-2 in the intricate regulation of prostanoid production and inflammatory signaling.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

Sf9 insect cells

Tag

C-His

Accession

P35354 (A18-L604)

Gene ID
Molecular Construction
N-term
COX-2 (A18-L604)
Accession # P35354
His
C-term
Synonyms
Prostaglandin G/H synthase 2; COX-2; PGHS-2
Molecular Weight

Approximately 66 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

COX-2 Protein, Human (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
COX-2 Protein, Human (sf9, His)
Cat. No.:
HY-P74222
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