CSNK1A1/Casein Kinase I alpha Protein, Human (Active, sf9, His, Strep)
Based on 1 Customer Validation
Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. CSNK1A1/Casein Kinase I alpha Protein, Human (sf9, His, Strep) is the recombinant human-derived CSNK1A1, expressed by Sf9 insect cells, with Strep, His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. CSNK1A1/Casein Kinase I alpha Protein, Human (sf9, His, Strep) is the recombinant human-derived CSNK1A1, expressed by Sf9 insect cells, with Strep, His labeled tag.
Background
Casein Kinase I alpha, a member of the casein kinase family, is characterized by its preference for acidic substrates such as caseins. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling by phosphorylating CTNNB1 at 'Ser-45.' Additionally, Casein Kinase I alpha may contribute to the phosphorylation of PER1 and PER2, suggesting a potential role in circadian rhythm regulation. Beyond its involvement in cellular signaling pathways, this kinase may play a crucial role in chromosome segregation during mitosis and regulate epithelial cell migration by influencing keratin cytoskeleton disassembly. Furthermore, Casein Kinase I alpha acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrient stimuli, mediating the phosphorylation of the DEPTOR inhibitor. In another context, it functions as an inhibitor of NLRP3 inflammasome assembly through the phosphorylation of NLRP3, highlighting its diverse roles in cellular processes.
Verified Bioactivity
The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 1.562 nM, the conversion rate is about 25%.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;C-StrepⅡ
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Accession
P48729-1 (A2-F337)
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Gene ID1452
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Protein Length
Full Length of Isoform-1
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Synonyms
CSNK1A1; CKIalpha; Casein Kinase 1 Alpha 1; Epididymis Secretory Sperm Binding Protein Li 77p; CK1; Non-Specific Serine/Threonine Protein Kinase; Casein Kinase I Isoform Alpha; Down-Regulated In Lung Cancer; CK1a; Casein Kinase 1, Alpha 1; CKIa; HEL-S-77p
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AA Sequence
ASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF
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Molecular Weight
Approximately 68 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 5 mM DTT, 0.1 M trehalose.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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