CutA Protein, Human (His)

CUTA protein is a trimeric membrane anchor protein of AChE and a homolog of the bacterial divalent cation chelator CutA1 protein. CUTA protein also interacts with BACE1 and inhibits APP beta processing and Aβ production. CutA Protein, Human (His) is the recombinant human-derived CutA protein, expressed by E. coli , with C-6*His labeled tag. The total length of CutA Protein, Human (His) is 156 a.a., with molecular weight of ~17.0 kDa.

CUTA 蛋白是哺乳动物脑乙酰胆碱酯酶 (AChE) 的膜锚蛋白，是一种三聚体蛋白，可能形成附着于乙酰胆碱酯酶 (AChE) 的膜蛋白复合物的一部分。人的 CUTA 与细菌 CutA1 蛋白同源，是二价阳离子耐受同源物。较长的 CutA 变体的表达会降低 AChE 的水平，这种效应取决于 AChE C 末端肽可能出现的错误折叠。CutA 增加了 C 末端具有 KDEL 基序的突变体的分泌；它还增加了 AChE 同源四聚体的形成。较长的 CutA 变体可能影响分泌蛋白的加工和运输，而较短的 CutA 变体可能在细胞质中具有独特的功能。例如，CUTA 蛋白与 β-分泌酶 β 位点 APP 裂解酶 1 (BACE1) 相互作用，并抑制 APP β 加工和 Aβ 生成。铜处理促进了 CUTA 下调诱导的 Aβ 分泌增加，但对 CUTA-β 位点 APP 裂解酶 1 相互作用没有影响。因此，说明铜和 CUTA 的相互调节，两者通过不同的机制调节 Aβ 的生成。

Human

E. coli

C-6*His

O60888-3 (M1-P156)

51596  [NCBI]

MPALLPVASRLLLLPRVLLTMASGSPPTQPSPASDSGSGYVPGSVSAAFVTCPNEKVAKEIARAVVEKRLAACVNLIPQITSIYEWKGKIEEDSEVLMMIKTQSSLVPALTDFVRSVHPYEVAEVIALPVEQGNFPYLQWVRQVTESVSDSITVLP

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 1mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.