DTD1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The DTD1 protein, as a potential ATPase, may participate in DNA replication by helping to load CDC45 onto the prereplication complex. In addition, it can also act as an aminoacyl-tRNA editing enzyme, specifically deacylating erroneous D-aminoacyl-tRNA and erroneous glycyl-tRNA (Ala). DTD1 Protein, Human (HEK293, His) is the recombinant human-derived DTD1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The DTD1 protein, as a potential ATPase, may participate in DNA replication by helping to load CDC45 onto the prereplication complex. In addition, it can also act as an aminoacyl-tRNA editing enzyme, specifically deacylating erroneous D-aminoacyl-tRNA and erroneous glycyl-tRNA (Ala). DTD1 Protein, Human (HEK293, His) is the recombinant human-derived DTD1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
DTD1 protein is a possible ATPase involved in DNA replication, potentially playing a role in the loading of CDC45 onto pre-replication complexes. Additionally, it functions as an aminoacyl-tRNA editing enzyme, specifically deacylating mischarged D-aminoacyl-tRNAs and mischarged glycyl-tRNA(Ala). Notably, the enzyme employs tRNA-based catalysis, rejecting L-amino acids rather than detecting D-amino acids in the active site. This activity aids in the recycling of D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, mitigating the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and contributing to the maintenance of protein L-homochirality.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q8TEA8 (M1-P209)
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Molecular Construction
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N-term
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DTD1 (M1-P209)
Accession # Q8TEA8 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
DTD1; BA379J5.3; Prev. C20orf88; DUE-B; Prev. HARS2; DTD; DUEB; D-Tyrosyl-TRNA Deacylase 1 Homolog (S. Cerevisiae); DNA-Unwinding Element-Binding Protein B; Chromosome 20 Open Reading Frame 88; Pqn-68; D-Tyrosyl-TRNA Deacylase 1 Homolog; Histidyl-TRNA Syn
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AA Sequence
MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFHLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP
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Predicted Molecular Mass
24.5 kDa
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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