DTD1 Protein, Human (HEK293, His)

The DTD1 protein, as a potential ATPase, may participate in DNA replication by helping to load CDC45 onto the prereplication complex. In addition, it can also act as an aminoacyl-tRNA editing enzyme, specifically deacylating erroneous D-aminoacyl-tRNA and erroneous glycyl-tRNA (Ala). DTD1 Protein, Human (HEK293, His) is the recombinant human-derived DTD1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of DTD1 Protein, Human (HEK293, His) is 209 a.a., with molecular weight of ~30.0 kDa.

DTD1 protein is a possible ATPase involved in DNA replication, potentially playing a role in the loading of CDC45 onto pre-replication complexes. Additionally, it functions as an aminoacyl-tRNA editing enzyme, specifically deacylating mischarged D-aminoacyl-tRNAs and mischarged glycyl-tRNA(Ala). Notably, the enzyme employs tRNA-based catalysis, rejecting L-amino acids rather than detecting D-amino acids in the active site. This activity aids in the recycling of D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, mitigating the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and contributing to the maintenance of protein L-homochirality.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

Q8TEA8 (M1-P209)

92675  [NCBI]

MKAVVQRVTRASVTVGGEQISAIGRGICVLLGISLEDTQKELEHMVRKILNLRVFEDESGKHWSKSVMDKQYEILCVSQFTLQCVLKGNKPDFHLAMPTEQAEGFYNSFLEQLRKTYRPELIKDGKFGAYMQVHIQNDGPVTIELESPAPGTATSDPKQLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSEREP

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.