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  3. Endosialin/CD248 Protein, Human (HEK293, His)

Endosialin/CD248 Protein, Human (HEK293, His)

Cat. No.: HY-P78042
COA Handling Instructions

Endosialin/CD248 Protein may contribute to tumor angiogenesis. Endosialin/CD248 Protein, Human (HEK293, His) is the recombinant human-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag. The total length of Endosialin/CD248 Protein, Human (HEK293, His) is 670 a.a., with molecular weight of 115-140 kDa.

For research use only. We do not sell to patients.

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Description

Endosialin/CD248 Protein may contribute to tumor angiogenesis. Endosialin/CD248 Protein, Human (HEK293, His) is the recombinant human-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag. The total length of Endosialin/CD248 Protein, Human (HEK293, His) is 670 a.a., with molecular weight of 115-140 kDa.

Background

Endosialin/CD248 protein is implicated in potential involvement in tumor angiogenesis. Its specific functions in this context may include interactions with processes related to the formation and development of blood vessels within tumors. The intricate role of Endosialin/CD248 in tumor angiogenesis suggests a contribution to the complex regulatory networks associated with vascularization in the context of tumorigenesis.

Biological Activity

Immobilized Human TEM1, His Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of <5.2 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q9HCU0-1 (Q18-L687)

Gene ID
Molecular Construction
N-term
Endosialin (Q18-L687)
Accession # Q9HCU0-1
His
C-term
Synonyms
Endosialin; CD248; CD164L1; TEM1; CD164 sialomucin-like 1; CD164L1; CD248 molecule; MGC119479
AA Sequence

QDPWAAEPRAACGPSSCYALFPRRRTFLEAWRACRELGGDLATPRTPEEAQRVDSLVGAGPASRLLWIGLQRQARQCQLQRPLRGFTWTTGDQDTAFTNWAQPASGGPCPAQRCVALEASGEHRWLEGSCTLAVDGYLCQFGFEGACPALQDEAGQAGPAVYTTPFHLVSTEFEWLPFGSVAAVQCQAGRGASLLCVKQPEGGVGWSRAGPLCLGTGCSPDNGGCEHECVEEVDGHVSCRCTEGFRLAADGRSCEDPCAQAPCEQQCEPGGPQGYSCHCRLGFRPAEDDPHRCVDTDECQIAGVCQQMCVNYVGGFECYCSEGHELEADGISCSPAGAMGAQASQDLGDELLDDGEDEEDEDEAWKAFNGGWTEMPGILWMEPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATHPTLPSAHQPPVIPATHPALSRDHQIPVIAANYPDLPSAYQPGILSVSHSAQPPAHQPPMISTKYPELFPAHQSPMFPDTRVAGTQTTTHLPGIPPNHAPLVTTLGAQLPPQAPDALVLRTQATQLPIIPTAQPSLTTTSRSPVSPAHQISVPAATQPAALPTLLPSQSPTNQTSPISPTHPHSKAPQIPREDGPSPKLALWLPSPAPTAAPTALGEAGLAEHSQRDDRWL

Molecular Weight

115-140 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Endosialin/CD248 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Endosialin/CD248 Protein, Human (HEK293, His)
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HY-P78042
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