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Endosialin/CD248 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Endosialin/CD248 Protein may contribute to tumor angiogenesis. Endosialin/CD248 Protein, Human (HEK293, His) is the recombinant human-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Endosialin/CD248 Protein, Human (HEK293, His)
  • Experimental Validation Results for Endosialin/CD248 Protein, Human (HEK293, His)
  • Endosialin/CD248 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Endosialin/CD248 Protein may contribute to tumor angiogenesis. Endosialin/CD248 Protein, Human (HEK293, His) is the recombinant human-derived Endosialin/CD248 protein, expressed by HEK293 , with C-His labeled tag.

Background

Endosialin/CD248 protein is implicated in potential involvement in tumor angiogenesis. Its specific functions in this context may include interactions with processes related to the formation and development of blood vessels within tumors. The intricate role of Endosialin/CD248 in tumor angiogenesis suggests a contribution to the complex regulatory networks associated with vascularization in the context of tumorigenesis.

Verified Bioactivity

1.Immobilized Human TEM1, His Tag at 0.5 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of <5.2 ng/mL determined by ELISA.
2.Immobilized Human Endosialin/CD248 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human Galectin-3BP/MAC-2BP. The ED50 for this effect is 0.3859 μg/mL.
3.Immobilized Human TEM1, His Tag at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 1.6ng/mL determined by ELISA.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 90%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Immobilized Human Endosialin/CD248 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human Galectin-3BP/MAC-2BP. The ED50 for this effect is 0.3859 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q9HCU0-1 (Q18-L687)

  • Gene ID
    57124  [NCBI]
  • Molecular Construction
    • N-term
    • Endosialin (Q18-L687)
      Accession # Q9HCU0-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD248; CD248 Molecule, Endosialin; Prev. CD164L1; CD164 Sialomucin-Like 1; Tumor Endothelial Marker 1; 2610111G01Rik; Endosialin; CD248 Antigen; TEM1; CD248 Molecule; CD248 Antigen, Endosialin

  • AA Sequence

    QDPWAAEPRAACGPSSCYALFPRRRTFLEAWRACRELGGDLATPRTPEEAQRVDSLVGAGPASRLLWIGLQRQARQCQLQRPLRGFTWTTGDQDTAFTNWAQPASGGPCPAQRCVALEASGEHRWLEGSCTLAVDGYLCQFGFEGACPALQDEAGQAGPAVYTTPFHLVSTEFEWLPFGSVAAVQCQAGRGASLLCVKQPEGGVGWSRAGPLCLGTGCSPDNGGCEHECVEEVDGHVSCRCTEGFRLAADGRSCEDPCAQAPCEQQCEPGGPQGYSCHCRLGFRPAEDDPHRCVDTDECQIAGVCQQMCVNYVGGFECYCSEGHELEADGISCSPAGAMGAQASQDLGDELLDDGEDEEDEDEAWKAFNGGWTEMPGILWMEPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATHPTLPSAHQPPVIPATHPALSRDHQIPVIAANYPDLPSAYQPGILSVSHSAQPPAHQPPMISTKYPELFPAHQSPMFPDTRVAGTQTTTHLPGIPPNHAPLVTTLGAQLPPQAPDALVLRTQATQLPIIPTAQPSLTTTSRSPVSPAHQISVPAATQPAALPTLLPSQSPTNQTSPISPTHPHSKAPQIPREDGPSPKLALWLPSPAPTAAPTALGEAGLAEHSQRDDRWL

  • Molecular Weight

    Approximately 125-155 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human Endosialin/CD248 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Human Galectin-3BP/MAC-2BP. The ED50 for this effect is 0.3859 μg/mL.

  • Endosialin/CD248 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Endosialin/CD248 Protein, Human (HEK293, His)
  • Experimental Validation Results for Endosialin/CD248 Protein, Human (HEK293, His)
  • Endosialin/CD248 Protein, Human (HEK293, His) Product Image