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Enolase 3/ENO3 Protein, Human (His)

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Enolase 3/ENO3 protein acts as a glycolytic enzyme, promoting the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its role in glycolysis, ENO3 appears to play a crucial role in the development and regeneration of striated muscle. Enolase 3/ENO3 Protein, Human (His) is the recombinant human-derived Enolase 3/ENO3 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Enolase 3/ENO3 protein acts as a glycolytic enzyme, promoting the conversion of 2-phosphoglycerate into phosphoenolpyruvate. In addition to its role in glycolysis, ENO3 appears to play a crucial role in the development and regeneration of striated muscle. Enolase 3/ENO3 Protein, Human (His) is the recombinant human-derived Enolase 3/ENO3 protein, expressed by E. coli , with N-His labeled tag.

Background

Enolase 3 (ENO3) is a glycolytic enzyme responsible for catalyzing the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. Beyond its classical role in energy metabolism, ENO3 appears to play a specific function in striated muscle development and regeneration. The enzyme's involvement in these processes suggests a role in supporting the energetic demands of muscle tissues during development and repair. This dual functionality highlights the multifaceted roles of ENO3 in both fundamental metabolic pathways and specialized cellular processes related to muscle development and regeneration.

Verified Bioactivity

Specific activity is 5235 pmol/min/µg, and was obtained by measuring the decrease of NAD that incubate at 37 ºC for 5 minutes in absorbance at 340 nm resulting from NADH at pH 6.5.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-10*His
  • Accession

    P13929-1/AAH17249.1 (M1-K434)

  • Gene ID
    2027  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • ENO3 (M1-K434)
      Accession # P13929-1/AAH17249.1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    ENO3; Muscle Enriched Enolase; Enolase 3; MSE; Muscle-Specific Enolase; 2-Phospho-D-Glycerate Hydrolyase; Skeletal Muscle Enolase; Enolase 3, (Beta, Muscle); Beta-Enolase; Phosphopyruvate Hydratase; 2-Phospho-D-Glycerate Hydro-Lyase; GSD13; Enolase 3 (Bet

  • AA Sequence

    MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKGVLKAVENINNTLGPALLQKKLSVVDQEKVDKFMIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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