EphA2 Protein, Human (Biotinylated, HEK293, His-Avi)
Based on 1 Customer Validation
The EphA2 protein is an important member of the ephrin receptor subfamily that mediates developmental events, particularly in the nervous system. It has a single kinase domain and an extracellular region that binds ephrin-A ligands. EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-6*His, C-Avi labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The EphA2 protein is an important member of the ephrin receptor subfamily that mediates developmental events, particularly in the nervous system. It has a single kinase domain and an extracellular region that binds ephrin-A ligands. EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-6*His, C-Avi labeled tag.
Background
EphA2 Protein, a member of the ephrin receptor subfamily within the protein-tyrosine kinase family, plays a crucial role in mediating developmental events, particularly within the nervous system. Featuring a single kinase domain and an extracellular region with a Cys-rich domain and 2 fibronectin type III repeats, EPH receptors are involved in binding ephrin-A and ephrin-B ligands. EphA2 specifically binds ephrin-A ligands and displays broad expression across various tissues, including esophagus (RPKM 37.8), urinary bladder (RPKM 18.3), and 21 other tissues. Additionally, mutations in this gene are associated with certain genetically-related cataract disorders, highlighting its significance in both developmental processes and ocular health.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized EFNA1-hFc at 10 μg/mL (100 μL/well) can bind Biotinylated EphA2 Protein, Human (HEK293, His-Avi) and the EC50 is 20-40 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His;C-Avi
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Accession
NP_004422.2 (A24-N534)
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Molecular Construction
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N-term
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EphA2 (A24-N534)
Accession # NP_004422.2 -
Avi-His
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C-term
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Protein Length
Extracellular Domain
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Conjugation
Biotin
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Synonyms
EPHA2; Protein Tyrosine Kinase; Prev. ECK; CTRCT6; Tyrosine-Protein Kinase Receptor ECK; EphA2; Ephrin Type-A Receptor 2; ARCC2; Epithelial Cell Receptor Protein Tyrosine Kinase; CTPP1; Receptor Protein-Tyrosine Kinase; CTPA; EPH Receptor A2; Epithelial C
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AA Sequence
AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN
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Predicted Molecular Mass
59.4 kDa
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Molecular Weight
Approximately 60-66 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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