Copy Link Print Information

EphB4 Protein, Human (HEK293)

1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

EphB4 protein is a receptor tyrosine kinase that binds to ephrin B ligand and initiates bidirectional signaling. Positive signaling regulates the rejection and separation of cells from EFNB2-expressing cells. EphB4 Protein, Human (HEK293) is the recombinant human-derived EphB4 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • EphB4 Protein, Human (HEK293) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

EphB4 protein is a receptor tyrosine kinase that binds to ephrin B ligand and initiates bidirectional signaling. Positive signaling regulates the rejection and separation of cells from EFNB2-expressing cells. EphB4 Protein, Human (HEK293) is the recombinant human-derived EphB4 protein, expressed by HEK293 , with tag free.

Background

EphB4 protein, a receptor tyrosine kinase, engages in promiscuous binding to transmembrane ephrin-B family ligands located on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is termed forward signaling, while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Collaborating with its cognate ligand, EFNB2, EphB4 is intricately involved in regulating cell adhesion and migration. Moreover, EphB4 assumes a central role in heart morphogenesis, angiogenesis, and the remodeling and permeability of blood vessels. The forward signaling mediated by EPHB4 is instrumental in controlling cellular repulsion and segregation from EFNB2-expressing cells.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    P54760-1/NP_004435.3 (L16-A539)

  • Gene ID
    2050  [NCBI]
  • Molecular Construction
    • N-term
    • EphB4 (L16-A539)
      Accession # P54760-1/NP_004435.3
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    EPHB4; Tyrosine-Protein Kinase Receptor HTK; Prev. HTK; Ephrin Receptor EphB4; Hepatoma Transmembrane Kinase; CMAVM2; Ephrin Type-B Receptor 4; LMPHM7; Tyro11; TYRO11; Tyrosine-Protein Kinase TYRO11; EphB4; MYK1; HFASD; EPH Receptor B4; Receptor Protein-T

  • AA Sequence

    LEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLA

  • Predicted Molecular Mass

    57.7 kDa

  • Molecular Weight

    Approximately 65-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • EphB4 Protein, Human (HEK293) Product Image
    1/16
  • EphB4 Protein, Human (HEK293) Product Image