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EphB4 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Ephb4 Protein, a receptor tyrosine kinase, plays a critical role in angiogenesis and development. Dysregulation of Ephb4 Protein has been associated with disorders like cancer and cardiovascular diseases. Targeting Ephb4 Protein may provide potential therapeutic interventions in these conditions by modulating angiogenesis, inhibiting tumor growth, and managing cardiovascular complications. EphB4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB4 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for EphB4 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for EphB4 Protein, Mouse (HEK293, His)
  • EphB4 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Ephb4 Protein, a receptor tyrosine kinase, plays a critical role in angiogenesis and development. Dysregulation of Ephb4 Protein has been associated with disorders like cancer and cardiovascular diseases. Targeting Ephb4 Protein may provide potential therapeutic interventions in these conditions by modulating angiogenesis, inhibiting tumor growth, and managing cardiovascular complications. EphB4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphB4 protein, expressed by HEK293 , with C-His labeled tag.

Background

Ephb4 Protein, a receptor tyrosine kinase, exhibits promiscuous binding to transmembrane ephrin-B ligands on neighboring cells, initiating contact-dependent bidirectional signaling. This leads to both forward signaling, downstream of the receptor, and reverse signaling, downstream of the ephrin ligand. Crucially, Ephb4 Protein, in conjunction with its cognate ligand EFNB2, regulates cell adhesion and migration, playing a pivotal role in heart morphogenesis, angiogenesis, blood vessel remodeling, and permeability. Notably, EPHB4-mediated forward signaling governs cellular repulsion and segregation from EFNB2-expressing cells.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Immobilized Recombinant Mouse EphB4 is immobilized at 2 µg/mL(100 µL/well) can bind Mouse Ephrin-B2 (HY-P73017). The ED50 for this effect is 1.803 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    P54761 (L16-A539)

  • Gene ID
    13846  [NCBI]
  • Molecular Construction
    • N-term
    • EphB4 (L16-A539)
      Accession # P54761-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    EPHB4; Tyrosine-Protein Kinase Receptor HTK; Prev. HTK; Ephrin Receptor EphB4; Hepatoma Transmembrane Kinase; CMAVM2; Ephrin Type-B Receptor 4; LMPHM7; Tyro11; TYRO11; Tyrosine-Protein Kinase TYRO11; EphB4; MYK1; HFASD; EPH Receptor B4; Receptor Protein-T

  • AA Sequence

    LEETLLNTKLETADLKWVTYPQAEGQWEELSGLDEEQHSVRTYEVCDMKRPGGQAHWLRTGWVPRRGAVHVYATIRFTMMECLSLPRASRSCKETFTVFYYESEADTATAHTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNIKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCSWLITNLTYFPETVPRELVVPVAGSCVANAVPTANPSPSLYCREDGQWAEQQVTGCSCAPGYEAAESNKVCRACGQGTFKPQIGDESCLPCPANSHSNNIGSPVCLCRIGYYRARSDPRSSPCTTPPSAPRSVVHHLNGSTLRLEWSAPLESGGREDLTYAVRCRECRPGGSCLPCGGDMTFDPGPRDLVEPWVAIRGLRPDVTYTFEVAALNGVSTLATGPPPFEPVNVTTDREVPPAVSDIRVTRSSPSSLILSWAIPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESESWREQLA

  • Molecular Weight

    Approximately 70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Recombinant Mouse EphB4 is immobilized at 2 µg/mL (100 µL/well) can bind Mouse Ephrin-B2 (HY-P73017). The ED50 for this effect is 1.803 ng/mL.

  • EphB4 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for EphB4 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for EphB4 Protein, Mouse (HEK293, His)
  • EphB4 Protein, Mouse (HEK293, His) Product Image