FAP Protein, Cynomolgus (HEK293, His)

FAP Protein is a type II transmembrane serine protease, a member of the propionyl peptidase family, that promotes cell invasion and tumor growth. FAP protein has the function of enhancing blood coagulation. FAP Protein is associated with a variety of human pathology, including fibrosis, arthritis, atherosclerosis, autoimmune diseases, metabolic diseases and cancer. FAP Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived FAP protein, expressed by HEK293 , with N-His labeled tag. The total length of FAP Protein, Cynomolgus (HEK293, His) is 731 a.a., with molecular weight of ~87 kDa.

FAP protein is a type II transmembrane serine protease that promotes cell invasion and tumor growth, and is almost completely expressed in pathological conditions such as fibrosis, arthritis, and cancer. FAP protein has dipeptidyl peptidase activity, thus being able to cleave neuropeptide Y, peptide YY, substance P, and brain natriuretic peptide. FAP protein can cleave α-2 anti-fibrinolytic enzyme, converting α2-anti-fibrinolytic enzyme into a more effective fibrinolytic inhibitor, with the function of enhancing coagulation. FAP protein promotes tumor growth through multiple mechanisms such as proliferation, invasion, angiogenesis, epithelial-mesenchymal transition, stem cell promotion, immune suppression, and drug resistance. FAP protein is associated with various human pathologies, including fibrosis, arthritis, atherosclerosis, autoimmune diseases, metabolic diseases, and cancer^[1][2].
Targeting FAP Protein to cancer-related fibroblasts can achieve highly specific imaging and treatment of solid tumors^[3].

Measured by its ability to convert the substrate benzyloxycarbonyl­Gly­Pro­7­amido­4­methylcoumarin (Z­GP­AMC) to Z­Gly­Pro and 7­amino­4­methylcoumarin (AMC) and the specific activity is >600 pmol/min/µg.

Cynomolgus

HEK293

N-His

XP_005573377.1 (R30-D760)

102134935  [NCBI]

RVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPFVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEDYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD

Approximately 87 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.