FGFR-1 beta Protein, Human (HEK293, His)

FGFR-1 beta Protein, Human (HEK293, His) is the recombinant human-derived FGFR-1 beta protein, expressed by HEK293, with C-His labeled tag.

FGFR-1 alpha, a tyrosine-protein kinase, functions as a cell-surface receptor for fibroblast growth factors and plays a pivotal role in regulating embryonic development, cell proliferation, differentiation, and migration. It is essential for normal mesoderm patterning, proper axial organization during embryonic development, skeletogenesis, and the development of the gonadotropin-releasing hormone (GnRH) neuronal system. Upon ligand binding, FGFR-1 alpha activates multiple signaling cascades, phosphorylating key proteins such as PLCG1, FRS2, GAB1, and SHB. This activation leads to the production of signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate through PLCG1. Moreover, phosphorylation of FRS2 triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, mediating the activation of RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. FGFR-1 alpha also promotes the phosphorylation of SHC1, STAT1, and PTPN11/SHP2. Within the nucleus, it enhances the activity of RPS6KA1 and CREB1, contributing to the regulation of transcription. The down-regulation of FGFR-1 alpha signaling occurs through IL17RD/SEF and FGFR-1 alpha ubiquitination, internalization, and degradation.

1.This product does not contain protein kinase domain.
2.Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED₅₀ for this effect is typically 100-600 ng/mL.

Human

HEK293

C-His

P11362-14/NP_075594.1 (R22-E285)

2260  [NCBI]

Partial

MWSWKCLLFWAVLVTATLCTARPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE

50-55 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.