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  4. FGFR-4/CD334 FGFR
  5. FGFR-4
  6. FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi)

FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78127
Handling Instructions

The FGFR-4 protein is a tyrosine protein kinase and cell surface receptor for fibroblast growth factor that regulates diverse cellular processes such as proliferation, differentiation, migration, lipid metabolism, and bile acid biosynthesis. Notably, it contributes to the downregulation of the bile acid synthase CYP7A1 in response to FGF19. FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi) is 348 a.a., with molecular weight of 55-72 kDa.

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Description

The FGFR-4 protein is a tyrosine protein kinase and cell surface receptor for fibroblast growth factor that regulates diverse cellular processes such as proliferation, differentiation, migration, lipid metabolism, and bile acid biosynthesis. Notably, it contributes to the downregulation of the bile acid synthase CYP7A1 in response to FGF19. FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived FGFR-4 protein, expressed by HEK293 , with C-His, C-Avi labeled tag. The total length of FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi) is 348 a.a., with molecular weight of 55-72 kDa.

Background

FGFR-4 protein is a tyrosine-protein kinase that functions as a cell-surface receptor for fibroblast growth factors. It plays a crucial role in regulating cell proliferation, differentiation, migration, lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. One of its important functions is to facilitate the down-regulation of CYP7A1, the key enzyme in bile acid synthesis, in response to FGF19. Upon ligand binding, FGFR-4 phosphorylates PLCG1 and FRS2, leading to the activation of various signaling cascades. This activation results in the production of diacylglycerol and inositol 1,4,5-trisphosphate, which are important cellular signaling molecules. Additionally, phosphorylation of FRS2 triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, leading to the activation of RAS, MAPK1/ERK2, MAPK3/ERK1, and the AKT1 signaling pathway. FGFR-4 also promotes the SRC-dependent phosphorylation of MMP14, a matrix protease, and facilitates its lysosomal degradation. The signaling of FGFR-4 is tightly regulated through receptor internalization and degradation, and MMP14 aids in this process. However, mutations that result in constitutive kinase activation or impair normal FGFR-4 inactivation can lead to aberrant signaling.

Species

Human

Source

HEK293

Tag

C-His;C-Avi

Accession

P22455 (L22-D369)

Gene ID
Molecular Construction
N-term
FGFR-4 (L22-D369)
Accession # P22455
His-Avi
C-term
Synonyms
CD334; FGF R4; FGFR4; FGFR-4; MGC20292; JTK2; TKF
Molecular Weight

55-72 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FGFR-4 Protein, Human (Biotinylated, HEK293, His-Avi)
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HY-P78127
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