FGFR-4 Protein, Rat (HEK293, His)

The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more.Its effects include downregulation of CYP7A1 in response to FGF19.FGFR-4 Protein, Rat (HEK293, His) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-His labeled tag.

FGFR-4 protein, a tyrosine-protein kinase, functions as a cell-surface receptor for fibroblast growth factors, playing a crucial role in the regulation of cell proliferation, differentiation, and migration. Additionally, it contributes to the control of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism, and phosphate homeostasis. Its significance is highlighted in the normal down-regulation of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Upon ligand binding, FGFR-4 activates various signaling cascades, including the phosphorylation of PLCG1 and FRS2. This activation leads to the production of cellular signaling molecules like diacylglycerol and inositol 1,4,5-trisphosphate. Furthermore, FRS2 phosphorylation triggers the recruitment of GRB2, GAB1, PIK3R1, and SOS1, activating RAS, MAPK1/ERK2, MAPK3/ERK1, the MAP kinase signaling pathway, and the AKT1 signaling pathway. Notably, FGFR-4 promotes SRC-dependent phosphorylation of the matrix protease MMP14, facilitating its lysosomal degradation. To regulate FGFR-4 signaling, the receptor undergoes internalization and degradation, a process facilitated by MMP14.

Rat

HEK293

C-His

Q498D6 (F17-D367)

25114  [NCBI]

Extracellular Domain

FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD

Approximately 64-68 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.