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GAS6 Protein, Human (630a.a, HEK293, His)

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Based on 1 publication(s) in Google Scholar

The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes. GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses. GAS6 Protein, Human (630a.a, HEK293, His) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes. GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses. GAS6 Protein, Human (630a.a, HEK293, His) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-His labeled tag.

Background

GAS6, a ligand for tyrosine-protein kinase receptors AXL, TYRO3, and MER, is implicated in diverse cellular processes including cell growth, survival, adhesion, and migration. GAS6/AXL signaling contributes to endothelial cell survival under acidic conditions by preventing apoptosis, facilitates optimal cytokine signaling during human natural killer cell development, plays a role in hepatic regeneration, influences gonadotropin-releasing hormone neuron survival and migration, regulates platelet activation, and modulates thrombotic responses. Additionally, in microbial infections, GAS6 can act as a bridge between virus envelope phosphatidylserine and the TAM receptor tyrosine kinase Axl, facilitating viral entry through apoptotic mimicry. This unique function extends to its involvement in the entry processes of diverse viruses, including Dengue, Vaccinia, ebolavirus, and marburgvirus, highlighting the multifaceted roles of GAS6 in cellular and infectious contexts.

Verified Bioactivity

1. Immobilized Human GAS6, His Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of ≤12.3 ng/mL determined by ELISA.
2. Immobilized Human GAS6, His Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 1.0-10.0 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q14393-2 (A49-A678)

  • Gene ID
    2621  [NCBI]
  • Molecular Construction
    • N-term
    • GAS6 (A49-A678)
      Accession # Q14393-2
    • 6*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    GAS6; AXSF; Prev. AXLLG; DKFZp666G247; AXL Receptor Tyrosine Kinase Ligand; FLJ34709; Growth Arrest-Specific Protein 6; GAS-6; Growth Arrest Specific 6; AXL Stimulatory Factor

  • AA Sequence

    AFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

  • Predicted Molecular Mass

    70.6 kDa

  • Molecular Weight

    Approximately 71-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as 0.22 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 8.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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